Electron spin resonance characterization of vascular xanthine and NAD(P)H oxidase activity in patients with coronary artery disease: Relation to endothelium-dependent vasodilation

Increased inactivation of nitric oxide by superoxide (O2*-) contributes to endothelial dysfunction in patients with coronary disease (CAD). We therefore characterized the vascular activities of xanthine oxidase and NAD(P)H oxidase, 2 major O2*--producing enzyme systems, and their relationship with f...

Full description

Saved in:
Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 2003-03, Vol.107 (10), p.1383-1389
Main Authors: SPIEKERMANN, Stephan, LANDMESSER, Ulf, DIKALOV, Sergey, BREDT, Martin, GAMEZ, Graciela, TATGE, Helma, REEPSCHLÄGER, Nina, HORNIG, Burkhard, DREXLER, Helmut, HARRISON, David G
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cardiology. Vascular system
Coronary Artery Disease - enzymology
Coronary Artery Disease - physiopathology
Coronary heart disease
Coronary Vessels - enzymology
Electron Spin Resonance Spectroscopy
Endothelium, Vascular - enzymology
Endothelium, Vascular - physiopathology
Heart
Humans
Hypercholesterolemia - enzymology
Hypercholesterolemia - physiopathology
Male
Medical sciences
Middle Aged
NADPH Oxidases - metabolism
Oxidative Stress
Superoxides - metabolism
Vasodilation
Xanthine Oxidase - metabolism
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Increased inactivation of nitric oxide by superoxide (O2*-) contributes to endothelial dysfunction in patients with coronary disease (CAD). We therefore characterized the vascular activities of xanthine oxidase and NAD(P)H oxidase, 2 major O2*--producing enzyme systems, and their relationship with flow-dependent, endothelium-mediated vasodilation (FDD) in patients with CAD. Xanthine- and NAD(P)H-mediated O*.- formation was determined in coronary arteries from 10 patients with CAD and 10 controls by using electron spin resonance spectroscopy. Furthermore, activity of endothelium-bound xanthine oxidase in vivo and FDD of the radial artery were determined in 21 patients with CAD and 10 controls. FDD was measured before and after infusion of the antioxidant vitamin C (25 mg/min i.a.) to determine the portion of FDD inhibited by radicals. In coronary arteries from patients with CAD, xanthine- and NAD(P)H-mediated O2*- formation was increased compared with controls (xanthine: 12+/-2 versus 7+/-1 nmol O2*-/ microg protein; NADH: 11+/-1 versus 7+/-1 nmol O2*-/ microg protein; and NADPH: 12+/-2 versus 9+/-1 nmol O2*-/ microg protein; each P200% in patients with CAD (25+/-4 versus 9+/-1 nmol O2*-/ microL plasma per min; P
ISSN:0009-7322
1524-4539
DOI:10.1161/01.CIR.0000056762.69302.46