Application of Immunoperoxidase-Based Techniques to Detect Herpesvirus Infection in Tortoises

Indirect (IIP) and direct (DIP) immunoperoxidase assays were developed for the serological and histological diagnoses of herpesvirus infection in tortoises, respectively. A mouse monoclonal antibody (MAb HL1546), specific for the heavy chain of tortoise IgY, was used as the secondary antibody in the...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2003-03, Vol.15 (2), p.133-140
Main Authors: Origgi, F.C, Klein, P.A, Tucker, S.J, Jacobson, E.R
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
antibodies
Antibodies, Viral - immunology
antigens
Antigens, Viral - analysis
Antigens, Viral - immunology
antiserum
Biological and medical sciences
cell culture
enzyme-linked immunosorbent assay
Herpesviridae - immunology
Herpesviridae - isolation & purification
Herpesviridae Infections - diagnosis
Herpesviridae Infections - veterinary
Immunoenzyme Techniques - veterinary
immunoglobulin Y
Infectious diseases
Medical sciences
mice
neutralization tests
sucrose
tortoises
Turtles - virology
Viral diseases
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Summary:Indirect (IIP) and direct (DIP) immunoperoxidase assays were developed for the serological and histological diagnoses of herpesvirus infection in tortoises, respectively. A mouse monoclonal antibody (MAb HL1546), specific for the heavy chain of tortoise IgY, was used as the secondary antibody in the IIP assay. Rabbit polyclonal antisera raised against 2 sucrose gradient–purified tortoise herpesvirus isolates (HV4295/7R/95 and HV1976) were used as primary antibodies for the detection of herpesvirus antigen either in infected cell cultures or in formalin-fixed, paraffin-embedded tissues. The IIP and DIP assays could detect either the presence of anti-herpesvirus antibody in the plasma of exposed tortoises or the presence of herpesvirus antigen in infected tissues, respectively. Although the IIP test complements the enzyme-linked immunosorbent assay and the serum neutralization test already available for measuring herpesvirus-specific antibody in tortoises, the DIP test is useful for the histological diagnosis of herpesvirus infection in tortoises.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870301500207