Compartmental fasciotomy and isolating a muscle from neighboring muscles interfere with myofascial force transmission within the rat anterior crural compartment

• Published in: Journal of morphology (1931) 2003-06, Vol.256 (3), p.306-321
• Main Authors: Huijing, Peter A., Maas, Huub, Baan, Guus C.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; anterior crural compartment; anterior tibial muscle; Biomechanical Phenomena; connective tissue; Connective Tissue - anatomy & histology; dissection; Electric Stimulation; extensor digitorum longus muscle; extensor hallucis longus muscle; Fascia - physiology; fasciotomy; Hindlimb - anatomy & histology; Hindlimb - physiology; length-force characteristics; Muscle Contraction - physiology; Muscle, Skeletal - physiology; myofascial force transmission; proximo-distal force difference; rat; Rats; Rats, Wistar - anatomy & histology; Rats, Wistar - physiology
• ISSN: 0362-2525; 1097-4687
• DOI: 10.1002/jmor.10097

Muscles within the anterior crural compartment (extensor digitorum longus, EDL; tibialis anterior, TA; and extensor hallucis longus, EHL) and within the peroneal compartment were excited simultaneously and maximally. All muscles were kept at constant length with the exception of EDL, for which muscle length was changed by moving its proximal tendon. Active and passive force was measured at proximal as well as distal EDL tendons and at the combined distal tendons of TA and EHL (TA+EHL). In the initial experimental condition, a difference (Fproximal > Fdistal) in EDL force, amounting to 0–14% of proximal force, was confirmed for most EDL lengths. This is interpreted as a clear proof of extramuscular myofascial force transmission, as no significant EDL length effects could be shown on TA+EHL force. Repeated measurements were confirmed to cause marked changes of both proximal and distal length–force characteristics, such as a shift of the whole ascending limb of the active curve, including optimum length, to higher lengths without decreasing optimum force, and decreasing active force at low lengths (by ∼57%). Repeated measurements also lowered proximal and distal EDL passive force (by up to 35%). The proximo–distal difference in passive as well as active EDL force was decreased, but persisted. At most lengths, this difference for active force amounted to a constant fraction (14%) of proximal force. TA+EHL force was not affected significantly. Subsequently, acute effects of experimental surgical alterations were studied: The first manipulation was full lateral fasciotomy of the anterior crural compartment that caused a further decrease in active force at the proximal EDL but not at the distal EDL tendon. Passive forces showed no further significant changes. The proximo–distal EDL active force difference decreased to 0–5% of proximal force. After fasciotomy, TA+EHL force increased by 30%. This was interpreted as evidence of increased intramuscular and decreased extramuscular myofascial force transmission. The second manipulation was full isolation of EDL from TA+EHL, but not from extramuscular connective tissues, which caused a further decrease of the EDL proximo–distal force differences, indicating a stiffening effect of the presence of TA+EHL on the extramuscular matrix. For EDL active force the difference was no longer significantly different from zero. In contrast, for EDL passive force the proximo–distal force difference persisted. It is concluded that extr