Cholesterol immobilization via ether-linked sepharose gels

Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 degrees C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns m...

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Bibliographic Details
Published in:Analyst (London) 1992-06, Vol.117 (6), p.953-957
Main Authors: SATSANGI, R. K, MOTT, G. E
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cholesterol - chemistry
Cholesterol - isolation & purification
Ethers
Fundamental and applied biological sciences. Psychology
Gels
Sepharose
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Summary:Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 degrees C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.
ISSN:0003-2654
1364-5528
DOI:10.1039/AN9921700953