Coextraction during reactive extraction of phenylalanine using Aliquat 336: Modeling extraction equilibrium

Reactive liquid–liquid extraction can be used to recover hydrophilic fermentation products that would not otherwise partition into nonpolar solvents through an ion‐exchange reaction at the two‐phase interface. However, the ion‐exchange reagent may not be specific to the solute of interest and other...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2003-06, Vol.82 (5), p.533-542
Main Authors: Pursell, Mark R., Mendes-Tatsis, M. Alcina, Stuckey, David C.
Format: Article
Language:English
Subjects:
Actinomycetales - chemistry
Actinomycetales - metabolism
Aliquat 336
Biological and medical sciences
Bioreactors
Biotechnology
Chromatography, Ion Exchange - methods
coextraction
Computer Simulation
equilibrium modeling
fermentation broth
Fundamental and applied biological sciences. Psychology
Hydroxides - isolation & purification
Hydroxides - metabolism
liquid-liquid extraction
Methods. Procedures. Technologies
Models, Biological
Models, Chemical
Others
phenylalanine
Phenylalanine - biosynthesis
Phenylalanine - isolation & purification
Potassium Compounds - isolation & purification
Potassium Compounds - metabolism
Quaternary Ammonium Compounds
Various methods and equipments
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Summary:Reactive liquid–liquid extraction can be used to recover hydrophilic fermentation products that would not otherwise partition into nonpolar solvents through an ion‐exchange reaction at the two‐phase interface. However, the ion‐exchange reagent may not be specific to the solute of interest and other compounds present may also be extracted. In this study, the effect on solute extraction of other compounds present in the extraction medium was investigated for phenylalanine extraction using Aliquat 336. The extent of extraction at equilibrium was modeled using the equilibrium constants for the reactions present in the process. The interaction of different species within a multicomponent medium was examined using the model and experimental results. It was found that the extent of extraction and coextraction is controlled by the thermodynamics of each extraction reaction and, due to the formation of a common product, the interaction between each of the reactions. The main competition to reactive extraction will come from hydrophobic anionic compounds that will be present in fermentation broth. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 533–542, 2003.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.10602