A highly conserved human gene encoding a novel member of WD-repeat family of proteins (WDR13)

We have identified and characterized a novel member of the WD-repeat motif gene family, WDR13, which contains 9 exons and 8 introns. The gene has been mapped to the genomic locus Xp11.23 by fluorescent in situ hybridization and in silico mapping. Sequence analysis has revealed a continuous open read...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2003-03, Vol.81 (3), p.315-328
Main Authors: Singh, Bhupendra N, Suresh, Amritha, UmaPrasad, Gogineni, Subramanian, Subbaya, Sultana, Mehar, Goel, Sandeep, Kumar, Satish, Singh, Lalji
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Alternate splicing
Alternative Splicing
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosomes, Human, X
Classical genetics, quantitative genetics, hybrids
Conserved Sequence
DNA, Complementary
Exons
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
In Situ Hybridization, Fluorescence
Introns
Molecular Sequence Data
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Open Reading Frames
Phylogeny
Regulatory protein
Sequence Homology, Amino Acid
Subcellular Fractions - metabolism
WD motif
WDR13
X chromosome
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Summary:We have identified and characterized a novel member of the WD-repeat motif gene family, WDR13, which contains 9 exons and 8 introns. The gene has been mapped to the genomic locus Xp11.23 by fluorescent in situ hybridization and in silico mapping. Sequence analysis has revealed a continuous open reading frame (ORF) encoding for 485 amino acids with six WD motifs. The expression of this gene has been detected in all the tissues analyzed with significantly varied expression levels among the tissues studied. Analysis of EST clones from various tissues, showing significant homology to WDR13, has identified two spliced variants. The transcription start point has been mapped. Promoter analysis has identified high activity in the 5′ UTR, which interestingly showed a testis-specific activity in the transgenic animals studied. The subcellular localization of the WDR13 protein in the nucleus suggests that it may also have a regulatory role in nuclear function along with protein-protein interaction like other members of the WD family of proteins.
ISSN:0888-7543
1089-8646
DOI:10.1016/S0888-7543(02)00036-8