Assembly of single-shelled cores and double-shelled virus-like particles after baculovirus expression of major structural proteins P3, P7 and P8 of Rice dwarf virus

1 Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan 2 Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan Correspondence Toshihiro Omura toomura{at}affrc.go.jp Expression of the core capsid protein P3 of Rice dwarf virus in a b...

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Bibliographic Details
Published in:Journal of general virology 2003-04, Vol.84 (4), p.981-984
Main Authors: Hagiwara, Kyoji, Higashi, Takahiko, Namba, Kazunori, Uehara-Ichiki, Tamaki, Omura, Toshihiro
Format: Article
Language:English
Subjects:
Animals
Baculoviridae - metabolism
Baculoviridae - ultrastructure
Baculovirus
Cell Line
Gene Expression
Genetic Vectors
Nucleocapsid Proteins - biosynthesis
Nucleocapsid Proteins - genetics
Recombinant Proteins - biosynthesis
Reoviridae - genetics
Rice dwarf virus
Viral Structural Proteins - biosynthesis
Viral Structural Proteins - genetics
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Summary:1 Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan 2 Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan Correspondence Toshihiro Omura toomura{at}affrc.go.jp Expression of the core capsid protein P3 of Rice dwarf virus in a baculovirus system resulted in the formation of single-shelled core-like particles in insect cells in the absence of any other capsid proteins. Double-shelled virus-like particles were also observed upon mixing or co-expression of P3 and the major outer capsid protein P8, suggesting that P3 and P8 have the ability to form double-shelled particles both in vivo and in vitro . Core protein P7 expressed in a similar manner was incorporated into the virus-like particles.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.18904-0