Effect of chronic Li+ treatment on free intracellular Mg2+ in human neuroblastoma SH-SY5Y cells

Objectives:  Previous findings have demonstrated Li+/Mg2+ competition at therapeutic intracellular Li+ levels after acute Li+ treatment in human neuroblastoma SH‐SY5Y cells. In the current study, we examined whether Li+/Mg2+ competition exists at therapeutically relevant extra‐ and intracellular [Li...

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Published in:Bipolar disorders 2003-02, Vol.5 (1), p.6-13
Main Authors: Abukhdeir, Abde M, Layden, Brian T, Minadeo, Nicole, Bryant, Fred B, Stubbs Jr, Evan B., De Freitas, Duarte Mota
Format: Article
Language:English
Subjects:
Analysis of Variance
atomic absorption
Bipolar Disorder - drug therapy
Bipolar Disorder - metabolism
Dose-Response Relationship, Drug
fluorescence
Humans
Intracellular Fluid - chemistry
Ion Transport - drug effects
lithium
Lithium - analysis
Lithium - pharmacology
magnesium
Magnesium - analysis
Magnesium - metabolism
Neuroblastoma
Tumor Cells, Cultured
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Summary:Objectives:  Previous findings have demonstrated Li+/Mg2+ competition at therapeutic intracellular Li+ levels after acute Li+ treatment in human neuroblastoma SH‐SY5Y cells. In the current study, we examined whether Li+/Mg2+ competition exists at therapeutically relevant extra‐ and intracellular [Li+] after chronic Li+ loading times. Methods:  In human neuroblastoma cells, intracellular free Mg2+ was determined by fluorescence spectroscopy with the fluorophore furaptra. Intracellular Li+ and Mg2+ were measured by atomic absorption spectrophotometry. Results:  After loading of the neuroblastoma cells with 1–2 mM extracellular Li+ for 24–72 h, the observed, increased intracellular free [Mg2+] levels were significantly higher (p
ISSN:1398-5647
1399-5618
DOI:10.1034/j.1399-5618.2003.02238.x