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Effect of chronic Li+ treatment on free intracellular Mg2+ in human neuroblastoma SH-SY5Y cells
Objectives: Previous findings have demonstrated Li+/Mg2+ competition at therapeutic intracellular Li+ levels after acute Li+ treatment in human neuroblastoma SH‐SY5Y cells. In the current study, we examined whether Li+/Mg2+ competition exists at therapeutically relevant extra‐ and intracellular [Li...
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| Published in: | Bipolar disorders 2003-02, Vol.5 (1), p.6-13 |
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| container_end_page | 13 |
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| container_title | Bipolar disorders |
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| creator | Abukhdeir, Abde M Layden, Brian T Minadeo, Nicole Bryant, Fred B Stubbs Jr, Evan B. De Freitas, Duarte Mota |
| description | Objectives: Previous findings have demonstrated Li+/Mg2+ competition at therapeutic intracellular Li+ levels after acute Li+ treatment in human neuroblastoma SH‐SY5Y cells. In the current study, we examined whether Li+/Mg2+ competition exists at therapeutically relevant extra‐ and intracellular [Li+] after chronic Li+ loading times.
Methods: In human neuroblastoma cells, intracellular free Mg2+ was determined by fluorescence spectroscopy with the fluorophore furaptra. Intracellular Li+ and Mg2+ were measured by atomic absorption spectrophotometry.
Results: After loading of the neuroblastoma cells with 1–2 mM extracellular Li+ for 24–72 h, the observed, increased intracellular free [Mg2+] levels were significantly higher (p |
| doi_str_mv | 10.1034/j.1399-5618.2003.02238.x |
| format | article |
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Methods: In human neuroblastoma cells, intracellular free Mg2+ was determined by fluorescence spectroscopy with the fluorophore furaptra. Intracellular Li+ and Mg2+ were measured by atomic absorption spectrophotometry.
Results: After loading of the neuroblastoma cells with 1–2 mM extracellular Li+ for 24–72 h, the observed, increased intracellular free [Mg2+] levels were significantly higher (p < 0.03) than those in matched Li+ free cells, and intracellular [Li+] was found to be at therapeutic intracellular levels (0.7–1.5 mM).
Conclusions: The results demonstrate that Li+/Mg2+ competition exists after chronic treatment with Li+ at therapeutically relevant intracellular Li+ levels in neuroblastoma cells. We found differences between acute and chronic Li+ treatment effects on the extent of Li+/Mg2+ competition. Possible reasons for these differences are discussed.</description><identifier>ISSN: 1398-5647</identifier><identifier>EISSN: 1399-5618</identifier><identifier>DOI: 10.1034/j.1399-5618.2003.02238.x</identifier><identifier>PMID: 12656932</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Analysis of Variance ; atomic absorption ; Bipolar Disorder - drug therapy ; Bipolar Disorder - metabolism ; Dose-Response Relationship, Drug ; fluorescence ; Humans ; Intracellular Fluid - chemistry ; Ion Transport - drug effects ; lithium ; Lithium - analysis ; Lithium - pharmacology ; magnesium ; Magnesium - analysis ; Magnesium - metabolism ; Neuroblastoma ; Tumor Cells, Cultured</subject><ispartof>Bipolar disorders, 2003-02, Vol.5 (1), p.6-13</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12656932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abukhdeir, Abde M</creatorcontrib><creatorcontrib>Layden, Brian T</creatorcontrib><creatorcontrib>Minadeo, Nicole</creatorcontrib><creatorcontrib>Bryant, Fred B</creatorcontrib><creatorcontrib>Stubbs Jr, Evan B.</creatorcontrib><creatorcontrib>De Freitas, Duarte Mota</creatorcontrib><title>Effect of chronic Li+ treatment on free intracellular Mg2+ in human neuroblastoma SH-SY5Y cells</title><title>Bipolar disorders</title><addtitle>Bipolar Disord</addtitle><description>Objectives: Previous findings have demonstrated Li+/Mg2+ competition at therapeutic intracellular Li+ levels after acute Li+ treatment in human neuroblastoma SH‐SY5Y cells. In the current study, we examined whether Li+/Mg2+ competition exists at therapeutically relevant extra‐ and intracellular [Li+] after chronic Li+ loading times.
Methods: In human neuroblastoma cells, intracellular free Mg2+ was determined by fluorescence spectroscopy with the fluorophore furaptra. Intracellular Li+ and Mg2+ were measured by atomic absorption spectrophotometry.
Results: After loading of the neuroblastoma cells with 1–2 mM extracellular Li+ for 24–72 h, the observed, increased intracellular free [Mg2+] levels were significantly higher (p < 0.03) than those in matched Li+ free cells, and intracellular [Li+] was found to be at therapeutic intracellular levels (0.7–1.5 mM).
Conclusions: The results demonstrate that Li+/Mg2+ competition exists after chronic treatment with Li+ at therapeutically relevant intracellular Li+ levels in neuroblastoma cells. We found differences between acute and chronic Li+ treatment effects on the extent of Li+/Mg2+ competition. Possible reasons for these differences are discussed.</description><subject>Analysis of Variance</subject><subject>atomic absorption</subject><subject>Bipolar Disorder - drug therapy</subject><subject>Bipolar Disorder - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>fluorescence</subject><subject>Humans</subject><subject>Intracellular Fluid - chemistry</subject><subject>Ion Transport - drug effects</subject><subject>lithium</subject><subject>Lithium - analysis</subject><subject>Lithium - pharmacology</subject><subject>magnesium</subject><subject>Magnesium - analysis</subject><subject>Magnesium - metabolism</subject><subject>Neuroblastoma</subject><subject>Tumor Cells, Cultured</subject><issn>1398-5647</issn><issn>1399-5618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpFkE1P3DAQhi1ExfdfqHzqBSW1x7HjXJD4hmoLh22pOI2c7ASyzQfYibr8-ya7lJ488vvondHDGJcilkIlX5exVFkWaSNtDEKoWAAoG6-22N5HsL2e7Tgn6S7bD2EphDQg9A7blWC0yRTsMbwsSyp63pW8ePZdWxV8Vh3z3pPrG2rHoOWlJ-JV23tXUF0PtfP8-xMcj1_8eWhcy1safJfXLvRd4_j8Jpo_6kc-weGQfSpdHejo_T1gP68uf5zfRLP769vz01lUgQIbLRQ4J4xRkOW2UEUGlOsCnJE5UbJQUiSlthqkTlXmhCiBnHW5Jp2YTAunDtiXTe-L714HCj02VZgucC11Q8BUSQWJsiP4-R0c8oYW-OKrxvk3_KdkBE42wJ-qprf_ucBJPS5xMoyTYZzU41o9rvDs4hbuYb0h2hRUoafVR4Hzv9GkKtX46-4aZw9za88uMvym_gJDhYUZ</recordid><startdate>200302</startdate><enddate>200302</enddate><creator>Abukhdeir, Abde M</creator><creator>Layden, Brian T</creator><creator>Minadeo, Nicole</creator><creator>Bryant, Fred B</creator><creator>Stubbs Jr, Evan B.</creator><creator>De Freitas, Duarte Mota</creator><general>Munksgaard International Publishers</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200302</creationdate><title>Effect of chronic Li+ treatment on free intracellular Mg2+ in human neuroblastoma SH-SY5Y cells</title><author>Abukhdeir, Abde M ; Layden, Brian T ; Minadeo, Nicole ; Bryant, Fred B ; Stubbs Jr, Evan B. ; De Freitas, Duarte Mota</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2328-d32aa066329b8c3c92eb5c2a61bee4d3104f585215739a00f2ea8ab5e546950a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analysis of Variance</topic><topic>atomic absorption</topic><topic>Bipolar Disorder - drug therapy</topic><topic>Bipolar Disorder - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>fluorescence</topic><topic>Humans</topic><topic>Intracellular Fluid - chemistry</topic><topic>Ion Transport - drug effects</topic><topic>lithium</topic><topic>Lithium - analysis</topic><topic>Lithium - pharmacology</topic><topic>magnesium</topic><topic>Magnesium - analysis</topic><topic>Magnesium - metabolism</topic><topic>Neuroblastoma</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abukhdeir, Abde M</creatorcontrib><creatorcontrib>Layden, Brian T</creatorcontrib><creatorcontrib>Minadeo, Nicole</creatorcontrib><creatorcontrib>Bryant, Fred B</creatorcontrib><creatorcontrib>Stubbs Jr, Evan B.</creatorcontrib><creatorcontrib>De Freitas, Duarte Mota</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Bipolar disorders</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abukhdeir, Abde M</au><au>Layden, Brian T</au><au>Minadeo, Nicole</au><au>Bryant, Fred B</au><au>Stubbs Jr, Evan B.</au><au>De Freitas, Duarte Mota</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of chronic Li+ treatment on free intracellular Mg2+ in human neuroblastoma SH-SY5Y cells</atitle><jtitle>Bipolar disorders</jtitle><addtitle>Bipolar Disord</addtitle><date>2003-02</date><risdate>2003</risdate><volume>5</volume><issue>1</issue><spage>6</spage><epage>13</epage><pages>6-13</pages><issn>1398-5647</issn><eissn>1399-5618</eissn><abstract>Objectives: Previous findings have demonstrated Li+/Mg2+ competition at therapeutic intracellular Li+ levels after acute Li+ treatment in human neuroblastoma SH‐SY5Y cells. In the current study, we examined whether Li+/Mg2+ competition exists at therapeutically relevant extra‐ and intracellular [Li+] after chronic Li+ loading times.
Methods: In human neuroblastoma cells, intracellular free Mg2+ was determined by fluorescence spectroscopy with the fluorophore furaptra. Intracellular Li+ and Mg2+ were measured by atomic absorption spectrophotometry.
Results: After loading of the neuroblastoma cells with 1–2 mM extracellular Li+ for 24–72 h, the observed, increased intracellular free [Mg2+] levels were significantly higher (p < 0.03) than those in matched Li+ free cells, and intracellular [Li+] was found to be at therapeutic intracellular levels (0.7–1.5 mM).
Conclusions: The results demonstrate that Li+/Mg2+ competition exists after chronic treatment with Li+ at therapeutically relevant intracellular Li+ levels in neuroblastoma cells. We found differences between acute and chronic Li+ treatment effects on the extent of Li+/Mg2+ competition. Possible reasons for these differences are discussed.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>12656932</pmid><doi>10.1034/j.1399-5618.2003.02238.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Analysis of Variance atomic absorption Bipolar Disorder - drug therapy Bipolar Disorder - metabolism Dose-Response Relationship, Drug fluorescence Humans Intracellular Fluid - chemistry Ion Transport - drug effects lithium Lithium - analysis Lithium - pharmacology magnesium Magnesium - analysis Magnesium - metabolism Neuroblastoma Tumor Cells, Cultured |
| title | Effect of chronic Li+ treatment on free intracellular Mg2+ in human neuroblastoma SH-SY5Y cells |
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