CpG island methylation of tumor-related genes in three primary central nervous system lymphomas in immunocompetent patients

• Published in: Cancer genetics and cytogenetics 2003-04, Vol.142 (1), p.21-24
• Main Authors: Gonzalez-Gomez, Pilar, Bello, M.Josefa, Arjona, Dolores, Alonso, M.Eva, Lomas, Jesus, Amiñoso, Cinthia, de Campos, Jose M., Sarasa, Jose L., Gutierrez, Manuel, Rey, Juan A.
• Format: Article
• Language: English
• Subjects: Aged; Apoptosis Regulatory Proteins; Biological and medical sciences; Brain - physiology; Calcium-Calmodulin-Dependent Protein Kinases - genetics; Central Nervous System Neoplasms - genetics; CpG Islands; Cyclin-Dependent Kinase Inhibitor p16 - genetics; Death-Associated Protein Kinases; DNA Methylation; DNA-Binding Proteins - genetics; Female; Genes, p53; Genes, Tumor Suppressor; Glutathione S-Transferase pi; Glutathione Transferase - genetics; Hematologic and hematopoietic diseases; Humans; Immunocompetence; Isoenzymes - genetics; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma - genetics; Male; Medical sciences; Middle Aged; Neurology; Nuclear Proteins - genetics; O-Methylguanine-DNA Methyltransferase - genetics; Promoter Regions, Genetic; Reference Values; Retinoblastoma Protein - genetics; Thrombospondin 1 - genetics; Tissue Inhibitor of Metalloproteinase-3 - genetics; Tumor Protein p73; Tumor Suppressor Protein p14ARF - genetics; Tumor Suppressor Proteins; Tumors of the nervous system. Phacomatoses
• ISSN: 0165-4608; 1873-4456
• DOI: 10.1016/S0165-4608(02)00799-9

We have determined the promoter CpG island methylation status of O 6-methylguanine-DNA methyltransferase ( MGMT), glutathione-S-transferase P1 ( GSTP1) , death-associated protein kinase ( DAPK) , p14 ARF, thrombospondin-1 ( THBS1) , tissue inhibitor of metalloproteinase-3 gene ( TIMP-3) , p73, p16 INK4A, RB1, and TP53 genes in three primary central nervous system lymphomas (PCNSL). Five genes ( GSTP1, DAPK, TIMP-3, p16 INK4A, and RB1) were hypermethylated in two samples, whereas MGMT, THBS1, and p73 were aberrantly methylated in only one sample. No case presented CpG island methylation for the p14 ARF and TP53 genes. These findings concur with previous data suggesting a frequent inactivation of p16 INK4A and very limited involvement of TP53 in PCNSL and also provide insights into the epigenetic molecular involvement of other tumor-related genes in this neoplasm.