Steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease

An adrenal-specific protein reacting with autoantibodies in the sera of patients with adult onset Addison's disease has been purified from human adrenal glands. The protein, mol.wt. 55K, has the biochemical characteristics of steroid 21-hydroxylase and reacts on Western blots with rabbit antibo...

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Bibliographic Details
Published in:FEBS letters 1992-08, Vol.309 (1), p.51-55
Main Authors: Bednarek, J., Furmaniak, J., Wedlock, N., Kiso, Y., Baumann-Antczak, A., Fowler, S., Krishnan, H., Craft, J.A., Smith, B.Rees
Format: Article
Language:English
Subjects:
21-Hydroxylase
Addison Disease - enzymology
Addison Disease - immunology
Addison's disease
Adrenal autoantibody
Adrenal Glands - immunology
Adult
Analytical, structural and metabolic biochemistry
Autoantibodies - analysis
Autoantigens - immunology
Autoimmunity
Biological and medical sciences
Blotting, Western
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Enzymes and enzyme inhibitors
Female
Fundamental and applied biological sciences. Psychology
Graves Disease - immunology
Humans
Immunoglobulin G - analysis
Immunoglobulin G - isolation & purification
man
Microsomes - immunology
Molecular Weight
Oxidoreductases
Placenta - immunology
Pregnancy
properties
purification
Saccharomyces cerevisiae - genetics
steroid 21-hydroxylase
Steroid 21-Hydroxylase - genetics
Steroid 21-Hydroxylase - immunology
Thyroid Gland - immunology
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Summary:An adrenal-specific protein reacting with autoantibodies in the sera of patients with adult onset Addison's disease has been purified from human adrenal glands. The protein, mol.wt. 55K, has the biochemical characteristics of steroid 21-hydroxylase and reacts on Western blots with rabbit antibodies to recombinant 21-hydroxylase. Absorption of the native human 55K adrenal protein with human adrenal autoantibodies prevented the subsequent reaction of the 55K protein with rabbit antibodies to 21-hydroxylase in Western blot analysis. In addition, human adrenal autoantibodies reacted with recombinant 21-hydroxylase expressed in yeast. These data indicate that the adrenal specific enzyme steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80737-2