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Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI

BACKGROUND: Meiotic spindles in living human oocytes can be visualized by the Polscope. This study investigated the relationship between the presence/location of the spindle in metaphase II (MII) oocytes and developmental competence of embryos in vitro. METHODS: The spindles in 626 MII oocytes were...

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Published in:Human reproduction (Oxford) 2003-04, Vol.18 (4), p.817-820
Main Authors: Moon, Jeong‐Hee, Hyun, Chang‐Seop, Lee, Seok‐Won, Son, Weon‐Young, Yoon, San‐Hyun, Lim, Jin‐Ho
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container_issue 4
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container_title Human reproduction (Oxford)
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creator Moon, Jeong‐Hee
Hyun, Chang‐Seop
Lee, Seok‐Won
Son, Weon‐Young
Yoon, San‐Hyun
Lim, Jin‐Ho
description BACKGROUND: Meiotic spindles in living human oocytes can be visualized by the Polscope. This study investigated the relationship between the presence/location of the spindle in metaphase II (MII) oocytes and developmental competence of embryos in vitro. METHODS: The spindles in 626 MII oocytes were examined by the Polscope and divided into six groups (A–F) based on the presence or absence of the spindles and the angle between the spindle and the first polar body. After ICSI, the fertilization and embryo development were evaluated. RESULTS: Meiotic spindles were imaged in 523 oocytes (83.5%), while 103 (16.5%) did not have a visible spindle (group F). The majority of oocytes (68.8%) had the spindle directly beneath or adjacent to the first polar body (groups A and B: 48.2 and 20.6%). Oocytes in group C (11.2%) had the spindle located between 60 and 120° angle away from the first polar body, those in group D (2.4%) had the spindle located between 120 and 180° angle and those in group E (1.1%) had the spindle located at 180° angle to the first polar body. The fertilization and embryonic development were similar in the oocytes with spindles regardless of spindle position. However, the rate of high quality embryos was significantly higher in the oocytes (64.2%) with visible spindles than in the oocytes (35.9%) without spindle and multipronuclear proportion showed a slight tendency to increase in oocytes without spindles. (10.7 versus 5.9%, P = 0.12; NS). CONCLUSIONS: the presence of a bi‐refringent meiotic spindle in human oocytes by using the Polscope can predict a higher embryonic developmental competence. However, the relative position of the spindle within the oocyte doesn’t appear to influence the developmental potential of embryos.
doi_str_mv 10.1093/humrep/deg165
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This study investigated the relationship between the presence/location of the spindle in metaphase II (MII) oocytes and developmental competence of embryos in vitro. METHODS: The spindles in 626 MII oocytes were examined by the Polscope and divided into six groups (A–F) based on the presence or absence of the spindles and the angle between the spindle and the first polar body. After ICSI, the fertilization and embryo development were evaluated. RESULTS: Meiotic spindles were imaged in 523 oocytes (83.5%), while 103 (16.5%) did not have a visible spindle (group F). The majority of oocytes (68.8%) had the spindle directly beneath or adjacent to the first polar body (groups A and B: 48.2 and 20.6%). Oocytes in group C (11.2%) had the spindle located between 60 and 120° angle away from the first polar body, those in group D (2.4%) had the spindle located between 120 and 180° angle and those in group E (1.1%) had the spindle located at 180° angle to the first polar body. The fertilization and embryonic development were similar in the oocytes with spindles regardless of spindle position. However, the rate of high quality embryos was significantly higher in the oocytes (64.2%) with visible spindles than in the oocytes (35.9%) without spindle and multipronuclear proportion showed a slight tendency to increase in oocytes without spindles. (10.7 versus 5.9%, P = 0.12; NS). CONCLUSIONS: the presence of a bi‐refringent meiotic spindle in human oocytes by using the Polscope can predict a higher embryonic developmental competence. However, the relative position of the spindle within the oocyte doesn’t appear to influence the developmental potential of embryos.</description><identifier>ISSN: 0268-1161</identifier><identifier>ISSN: 1460-2350</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/deg165</identifier><identifier>PMID: 12660277</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adult ; Biological and medical sciences ; Birefringence ; Cell Survival ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development - physiology ; Female ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; Humans ; Key words: embryo development/ICSI/MII oocytes/Polscope/spindle ; Metaphase ; Oocytes - physiology ; Oocytes - ultrastructure ; Prognosis ; Sperm Injections, Intracytoplasmic ; Spindle Apparatus - ultrastructure</subject><ispartof>Human reproduction (Oxford), 2003-04, Vol.18 (4), p.817-820</ispartof><rights>2003</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Apr 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-7f72b630e07e672870886e36022b5369ef25669a85f9b76d9b7921436a6f821b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=14919393$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12660277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moon, Jeong‐Hee</creatorcontrib><creatorcontrib>Hyun, Chang‐Seop</creatorcontrib><creatorcontrib>Lee, Seok‐Won</creatorcontrib><creatorcontrib>Son, Weon‐Young</creatorcontrib><creatorcontrib>Yoon, San‐Hyun</creatorcontrib><creatorcontrib>Lim, Jin‐Ho</creatorcontrib><title>Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: Meiotic spindles in living human oocytes can be visualized by the Polscope. This study investigated the relationship between the presence/location of the spindle in metaphase II (MII) oocytes and developmental competence of embryos in vitro. METHODS: The spindles in 626 MII oocytes were examined by the Polscope and divided into six groups (A–F) based on the presence or absence of the spindles and the angle between the spindle and the first polar body. After ICSI, the fertilization and embryo development were evaluated. RESULTS: Meiotic spindles were imaged in 523 oocytes (83.5%), while 103 (16.5%) did not have a visible spindle (group F). The majority of oocytes (68.8%) had the spindle directly beneath or adjacent to the first polar body (groups A and B: 48.2 and 20.6%). 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Segmentation. Gastrulation. Neurulation</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. 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Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>18</volume><issue>4</issue><spage>817</spage><epage>820</epage><pages>817-820</pages><issn>0268-1161</issn><issn>1460-2350</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: Meiotic spindles in living human oocytes can be visualized by the Polscope. This study investigated the relationship between the presence/location of the spindle in metaphase II (MII) oocytes and developmental competence of embryos in vitro. METHODS: The spindles in 626 MII oocytes were examined by the Polscope and divided into six groups (A–F) based on the presence or absence of the spindles and the angle between the spindle and the first polar body. After ICSI, the fertilization and embryo development were evaluated. RESULTS: Meiotic spindles were imaged in 523 oocytes (83.5%), while 103 (16.5%) did not have a visible spindle (group F). The majority of oocytes (68.8%) had the spindle directly beneath or adjacent to the first polar body (groups A and B: 48.2 and 20.6%). Oocytes in group C (11.2%) had the spindle located between 60 and 120° angle away from the first polar body, those in group D (2.4%) had the spindle located between 120 and 180° angle and those in group E (1.1%) had the spindle located at 180° angle to the first polar body. The fertilization and embryonic development were similar in the oocytes with spindles regardless of spindle position. However, the rate of high quality embryos was significantly higher in the oocytes (64.2%) with visible spindles than in the oocytes (35.9%) without spindle and multipronuclear proportion showed a slight tendency to increase in oocytes without spindles. (10.7 versus 5.9%, P = 0.12; NS). CONCLUSIONS: the presence of a bi‐refringent meiotic spindle in human oocytes by using the Polscope can predict a higher embryonic developmental competence. However, the relative position of the spindle within the oocyte doesn’t appear to influence the developmental potential of embryos.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>12660277</pmid><doi>10.1093/humrep/deg165</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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ispartof Human reproduction (Oxford), 2003-04, Vol.18 (4), p.817-820
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1460-2350
1460-2350
language eng
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source Oxford Journals Online
subjects Adult
Biological and medical sciences
Birefringence
Cell Survival
Early stages. Segmentation. Gastrulation. Neurulation
Embryology: invertebrates and vertebrates. Teratology
Embryonic and Fetal Development - physiology
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Humans
Key words: embryo development/ICSI/MII oocytes/Polscope/spindle
Metaphase
Oocytes - physiology
Oocytes - ultrastructure
Prognosis
Sperm Injections, Intracytoplasmic
Spindle Apparatus - ultrastructure
title Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI
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