Pyridoacridine Alkaloids Inducing Neuronal Differentiation in a Neuroblastoma Cell Line, from Marine Sponge Biemna fortis

• Published in: Bioorganic & medicinal chemistry 2003-05, Vol.11 (9), p.1969-1973
• Main Authors: Aoki, Shunji, Wei, Hong, Matsui, Kouhei, Rachmat, Rachmaniar, Kobayashi, Motomasa
• Format: Article
• Language: English
• Subjects: Acridines - chemistry; Acridines - isolation & purification; Acridines - pharmacology; Alkaloids - chemistry; Alkaloids - isolation & purification; Alkaloids - pharmacology; Animals; Biological and medical sciences; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cell Line, Tumor - cytology; Cell Line, Tumor - drug effects; Cell Transformation, Neoplastic - drug effects; Cell Transformation, Neoplastic - pathology; General pharmacology; Medical sciences; Mice; Neuroblastoma; Neurons - cytology; Neurons - drug effects; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenanthrolines - chemistry; Phenanthrolines - isolation & purification; Phenanthrolines - pharmacology; Porifera
• ISSN: 0968-0896; 1464-3391
• DOI: 10.1016/S0968-0896(03)00086-5

A new and three known pyridoacridine alkaloids were isolated from the Indonesian marine sponge Biemna fortis as neuronal differentiation inducers against a murine neuroblastoma cell line, Neuro 2A. The chemical structure of the new compound, labuanine A ( 1), was determined by spectroscopic study and chemical conversion. These pyridoacridine alkaloids induced multipolar neuritogenesis in more than 50% of cells at 0.03–3 μM concentration. Compound 3, which showed the strongest neuritogenic activity among them, also induced increase of acetylcholinesterase, a neuronal marker in Neuro 2A and arrested cell cycle at the G2/M phase. Labuanine A ( 1), a novel pyridoacridine alkaloid and three known related compounds 2– 4 were isolated from marine sponge Bienna fortis as neuronal differentiation inducers against a murine neuroblastoma cell line, Neuro 2A.