Promoter methylation and progressive transgene inactivation in Arabidopsis

Agrobacterium-transformed Arabidopsis plants were generated and the stability of their T-DNA-encoded resistance to kanamycin was examined. Of seven families, each homozygous for a single insertion event, two showed progressive inactivation of resistance over four generations of inbreeding. Loss of r...

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Bibliographic Details
Published in:Plant molecular biology 1992-10, Vol.20 (1), p.103-112
Main Authors: Kilby, N.J. (Cambridge Univ. (United Kingdom). Dept. of Genetics), Leyser, H.M.O, Furner, I.J
Format: Article
Language:English
Subjects:
ADN
AGROBACTERIUM
Arabidopsis
Biological and medical sciences
CHEMICAL RESISTANCE
CRUCIFERAE
DNA
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
DNA, Bacterial - metabolism
DNA, Ribosomal - genetics
DNA, Ribosomal - metabolism
Drug Resistance - genetics
Fundamental and applied biological sciences. Psychology
GENE
GENES
Inbreeding
KANAMICINA
KANAMYCIN
Kanamycin - pharmacology
Kanamycin Kinase
KANAMYCINE
METHYLATION
METILACION
Molecular and cellular biology
Molecular genetics
Phosphotransferases - genetics
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants - drug effects
Plants - genetics
Plants, Genetically Modified
Plasmids
Promoter Regions, Genetic
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTENCIA QUIMICA
Restriction Mapping
TRANSGENIC PLANTS
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Summary:Agrobacterium-transformed Arabidopsis plants were generated and the stability of their T-DNA-encoded resistance to kanamycin was examined. Of seven families, each homozygous for a single insertion event, two showed progressive inactivation of resistance over four generations of inbreeding. Loss of resistance was associated with methylation of an Sst II site in the nos promoter of the kanamycin resistance gene. Treatment of plant roots from inactive lines with the demethylating agent 5-azacytidine restored the ability of such lines to form callus on kanamycin-containing media. These observations are consistent with the view that methylation is a factor in the progressive inactivation of transgenes in Arabidopsis.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00029153