Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana: Responses to nitrate provision

Four low-affinity (NRT1), and seven high-affinity (NRT2) nitrate transporter gene homologues have been identified in Arabidopsis thaliana. We investigated the transcript abundances of all eleven genes in shoot and root tissues in response to the provision of 1 mM NO3–, using relative quantitative RT...

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Bibliographic Details
Published in:Plant and cell physiology 2003-03, Vol.44 (3), p.304-317
Main Authors: Okamoto, M. (University of British Columbia, Vancouver (Canada)), Vidmar, J.J, Glass, A.D.M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anion Transport Proteins - genetics
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis Proteins - genetics
ARABIDOPSIS THALIANA
casein kinase 2
CK2
GENE EXPRESSION
Gene Expression Regulation, Plant - drug effects
GENES
HATS
high-affinity transporter system
Keywords: Arabidopsis thaliana — Gene expression — HATS/LATS — Nitrate transporter — NRT — 13NO3
LATS
low-affinity transporter system
major facilitator superfamily
MFS
Molecular Sequence Data
Multigene Family - genetics
nitrate transporter
nitrate/nitrite porter
Nitrates - pharmacology
NITROGEN METABOLISM
NNP
NRT
PKC
Plant Proteins
protein kinase C
Reverse Transcriptase Polymerase Chain Reaction
reverse transcription-PCR
RT-PCR
Sequence Homology, Amino Acid
TMS
trans-membrane-spanners
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Summary:Four low-affinity (NRT1), and seven high-affinity (NRT2) nitrate transporter gene homologues have been identified in Arabidopsis thaliana. We investigated the transcript abundances of all eleven genes in shoot and root tissues in response to the provision of 1 mM NO3–, using relative quantitative RT-PCR. Based upon this criterion, genes were classified as nitrate-inducible, nitrate-repressible, or nitrate-constitutive. AtNRT1.1, 2.1, and 2.2 were strongly induced by NO3–, peaking at 3–12 h and subsequently declining. By contrast AtNRT2.4 showed only modest induction both in shoots and roots. Expression of AtNRT2.5, one of the nitrate-repressible genes, was strongly suppressed by nitrate provision in both roots and shoots. The last group, characterized by a constitutive expression pattern, included AtNRT1.2, 1.4, 2.3, 2.6, and 2.7. Correlation coefficients between 13NO3– influx from 100 µM and 5 mM [NO3–], suggest that high- and low-affinity transport systems are mediated primarily by AtNRT2.1 and AtNRT1.1, respectively. Functional roles for the other members of these families remain uncertain.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcg036