Carbohydrates in mammalian tryptophanyl-tRNA synthetase

Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5–3.0% (w/w) of the enzyme composed of two subunits (60 kDa × 2). The same set of sug...

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Bibliographic Details
Published in:FEBS letters 1992-09, Vol.309 (3), p.337-339
Main Authors: Kovaleva, Galina K., Zheltova, Anna O., Nikitushkina, Tatyana V., Egorov, Tsezi A., Musoljamov, Aleksander Ch, Kisselev, Lev L.
Format: Article
Language:English
Subjects:
2-ME
2-mercaploethanol
Aminoacyl-tRNA synthetase
ammonium sulfate
AmS
Analytical, structural and metabolic biochemistry
Animals
Beef
Biological and medical sciences
bovine tryptophanyl-tRNA synthetase
Carbohydrate Metabolism
Cattle
Chromatography, Gel
dithiotreitol
DTT
Electrophoresis, Polyacrylamide Gel
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Glycoprotein
Ligases
Mammal
Sugar
TFA
trifluoroacetic acid
Tryptophan-tRNA Ligase - isolation & purification
Tryptophan-tRNA Ligase - metabolism
Tryptophanyl-tRNA synthetase
WRS
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Summary:Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5–3.0% (w/w) of the enzyme composed of two subunits (60 kDa × 2). The same set of sugars was detected in clastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80802-N