Effect of reduced temperatures and brefeldin A on prolactin secretion and on subcellular distribution of the secretory product and membrane antigens in GH3 pituitary cells

The effects of reduced temperatures (20, 15 or 10°C) and brefeldin A (BFA) on prolactin (PRL) secretion in the GH3 rat pituitary cell line have been compared. Both treatments inhibit PRL release to different extents. Ultrastructural immunocytochemistry reveals that, depending on the treatment, PRL i...

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Bibliographic Details
Published in:Biology of the cell 1992, Vol.75 (1), p.25-35
Main Authors: Nasciutti, Luiz-Eurico, Picart, Rene´e, Rosenbaum, Etelka, Tixier-Vidal, Andre´e, Tougard, Claude
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface - metabolism
Biological Transport, Active - drug effects
Brefeldin A
Clone Cells - drug effects
Clone Cells - physiology
Clone Cells - ultrastructure
Cold Temperature
Cyclopentanes - pharmacology
Endoplasmic Reticulum - drug effects
Endoplasmic Reticulum - immunology
Golgi Apparatus - drug effects
Golgi Apparatus - immunology
immunocytochemistry
Microscopy, Electron
Pituitary Gland - drug effects
Pituitary Gland - physiology
Pituitary Gland - ultrastructure
prolactin
Prolactin - metabolism
Prolactin - secretion
reduced temperatures
secretion
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Summary:The effects of reduced temperatures (20, 15 or 10°C) and brefeldin A (BFA) on prolactin (PRL) secretion in the GH3 rat pituitary cell line have been compared. Both treatments inhibit PRL release to different extents. Ultrastructural immunocytochemistry reveals that, depending on the treatment, PRL is blocked at different steps during its intracellular transit. The temperatures of 20 and 15°C block the PRL transport at one face of the Golgi stacks whereas both the temperature of 10°C and BFA treatment induce an arrest of PRL at the level of the rough endoplasmic reticulum (RER) cisternae. Moreover, exposure to 10°C or BFA induces an accumulation of a specific Golgi membrane antigen in the dilated RER structures. However, although disorganized and no longer definable under BFA treatment, the Golgi apparatus remains visible at 10°C. These two last treatments cause also an increase in the number of partly rough, partly smooth tubular structures tentatively called ‘paired cisternae’.
ISSN:0248-4900
1768-322X
DOI:10.1016/0248-4900(92)90121-G