Protein kinase and protein phosphatase expression in amyotrophic lateral sclerosis spinal cord

• Published in: Journal of neurochemistry 2003-04, Vol.85 (2), p.432-442
• Main Authors: Hu, J.‐H., Zhang, H., Wagey, R., Krieger, C., Pelech, S. L.
• Format: Article
• Language: English
• Subjects: Aged; amyotrophic lateral sclerosis; Amyotrophic Lateral Sclerosis - enzymology; Amyotrophic Lateral Sclerosis - pathology; Animals; Biological and medical sciences; CaM kinase I activator; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Enzyme Stability; expression; extracellular signal-regulated kinase 2; Freezing; Glycogen Synthase Kinase 3 - analysis; Glycogen Synthase Kinase 3 - biosynthesis; Humans; Isoenzymes - analysis; Isoenzymes - biosynthesis; Medical sciences; Neurology; phosphoprotein; Phosphoprotein Phosphatases - analysis; Phosphoprotein Phosphatases - biosynthesis; phosphorylation; Postmortem Changes; protein kinase; Protein Kinase C - analysis; Protein Kinase C - biosynthesis; Protein Kinases - analysis; Protein Kinases - biosynthesis; protein phosphatase; Protein Phosphatase 1; Rats; Rats, Sprague-Dawley; Spinal Cord - chemistry; Spinal Cord - enzymology; Spinal Cord - pathology; stress-activated protein kinase; Time Factors
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1046/j.1471-4159.2003.01670.x

The Kinetworks™ multi‐immunoblotting technique was used to evaluate the expressions of 78 protein kinases, 24 protein phosphatases and phosphorylation states of 31 phosphoproteins in thoracic spinal cord tissue from control subjects and patients having the sporadic form of amyotrophic lateral sclerosis (ALS). In both the cytosolic (C) and particulate (P) fractions of spinal cord from ALS patients as compared with controls, there were increased levels of calcium/calmodulin‐dependent protein kinase kinase (CaMKK; C = 120% increase/P = 580% increase;% change, compared with control), extracellular regulated kinase 2 (ERK2; C = 120% increase/P = 170% increase), G protein‐coupled receptor kinase 2 (GRK2; C = 140% increase/P = 140% increase), phospho‐Y279/216 glycogen synthase kinase 3 α/β (GSK3α/β; C = 90% increase/P = 220% increase), protein kinase B α (PKBα; C = 360% increase/P = 200% increase), phospho‐T638 PKCα/β (C = 630% increase/P = 170% increase), cGMP‐dependent protein kinase (PKG; C = 100% increase/P = 75% increase), phospho‐T451 dsRNA‐dependent protein kinase (PKR; C = 2600% increase/P = 3330% increase), ribosomal S6 kinase 1 (RSK1; C = 750% increase/P = 630% increase), phospho‐T389 p70 S6 kinase (S6K; C = 1000% increase/P = 460% increase), and protein‐tyrosine phosphatase 1 δ (PTP1δ; C = 43% increase/P = 70% increase). Cytosolic increases in phospho‐α‐S724/γ‐S662 adducin (C = 15650% increase), PKCα (C = 100% increase) and PKCζ (C = 190% increase) were found in ALS patients as compared with controls, while particulate increases in cAMP‐dependent protein kinase (PKA; 43% increase), protein kinase C β (PKCβ; 330% increase), and stress‐activated protein kinase β (SAPKβ; 34% increase) were also observed. Cyclin‐dependent kinase‐associated phosphatase (KAP) was apparently translocated, as it was reduced (31% decrease) in cytosolic fractions but elevated (100% increase) in particulate fractions of ALS spinal cord tissue. Our observations indicate that ALS is associated with the elevated expression and/or activation of many protein kinases, including PKCα, PKCβ, PKCζ and GSK3α/β, which may augment neural death in ALS, and CaMKK, PKBα, Rsk1, S6K, and SAPK, which may be a response to neuronal injury that potentially can mitigate cell death.