Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations

Excessive degradation of the vascular matrix by matrix metalloproteinases (MMPs) can lead to structural instability of vessels. In this study we examined the expression of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in brain arteriovenous malformations (BAVMs). We performed gelatin zymo...

Full description

Saved in:
Bibliographic Details
Published in:Stroke (1970) 2003-04, Vol.34 (4), p.925-931
Main Authors: Hashimoto, Tomoki, Wen, Gen, Lawton, Michael T, Boudreau, Nancy J, Bollen, Andrew W, Yang, Guo-Yuan, Barbaro, Nicholas M, Higashida, Randall T, Dowd, Christopher F, Halbach, Van V, Young, William L
Format: Article
Language:English
Subjects:
Adult
Brain - enzymology
Embolization, Therapeutic
Female
Humans
Intracranial Arteriovenous Malformations - diagnosis
Intracranial Arteriovenous Malformations - enzymology
Intracranial Arteriovenous Malformations - therapy
Intracranial Hemorrhages - diagnosis
Leukocyte Common Antigens - metabolism
Male
Matrix Metalloproteinase 9 - metabolism
Matrix Metalloproteinases - metabolism
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Tissue Inhibitor of Metalloproteinases - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Excessive degradation of the vascular matrix by matrix metalloproteinases (MMPs) can lead to structural instability of vessels. In this study we examined the expression of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in brain arteriovenous malformations (BAVMs). We performed gelatin zymography for MMPs and Western blot for MMP-9, MMP-2, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. MMP-9 expression was localized by immunohistochemistry. We analyzed 37 BAVM specimens and 9 control brain specimens from epilepsy surgery. Thirty-two BAVM patients had embolization treatment before resection. Eighteen BAVM patients had a history of hemorrhage from BAVMs. Neither MMP-2 nor TIMP-2 was detected in BAVMs or control brain specimens. Compared with control brain samples, BAVM samples had higher levels of total MMP-9, active MMP-9, pro-MMP-9, TIMP-1, and TIMP-3. TIMP-4 levels were higher in the control brain than in BAVM specimens. MMP-9 was localized to the endothelial cell/peri-endothelial cell layer and infiltrating neutrophils of BAVMs. BAVMs with venous stenosis >or=50% had higher expression of MMP-9 than BAVMs with venous stenosis
ISSN:0039-2499
1524-4628
DOI:10.1161/01.str.0000061888.71524.df