Interleukin-10 receptor expression in systemic lupus erythematosus and rheumatoid arthritis

We aimed to determine the expression of the interleukin-10 receptor (IL10R) on circulating leukocytes in SLE and rheumatoid arthritis, and correlate this with plasma IL-10 levels and disease activity. Peripheral blood was sampled from 20 SLE patients, 14 rheumatoid arthritis patients, and 14 healthy...

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Bibliographic Details
Published in:Clinical and experimental rheumatology 2003, Vol.21 (1), p.83-86
Main Authors: CAIRNS, A. P, CROCKARD, A. D, BELL, A. L
Format: Article
Language:English
Subjects:
Arthritis, Rheumatoid - blood
Arthritis, Rheumatoid - physiopathology
Biological and medical sciences
Diseases of the osteoarticular system
Female
Flow Cytometry
Fluorescent Antibody Technique
Humans
Inflammatory joint diseases
Leukocytes - metabolism
Lupus Erythematosus, Systemic - blood
Lupus Erythematosus, Systemic - physiopathology
Male
Medical sciences
Middle Aged
Receptors, Interleukin - blood
Receptors, Interleukin-10
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Severity of Illness Index
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Summary:We aimed to determine the expression of the interleukin-10 receptor (IL10R) on circulating leukocytes in SLE and rheumatoid arthritis, and correlate this with plasma IL-10 levels and disease activity. Peripheral blood was sampled from 20 SLE patients, 14 rheumatoid arthritis patients, and 14 healthy controls. IL-10R expression was determined by immunofluorescence labelling and flow cytometric analysis. Plasma IL-10 levels were measured by ELISA. IL-10R was highly expressed on monocytes, and to a lesser degree on neutrophils in all 3 patient groups. Only a small percentage of lymphocytes expressed IL-10R in all three groups. There was no significant difference in IL-10R expression on the surface of monocytes, neutrophils or lymphocytes in any of the 3 groups. IL-10R expression did not correlate with plasma IL-10 levels or disease activity. This study has shown no difference in surface IL-10R expression between SLE, rheumatoid arthritis and normal subjects. Deficient or excessive circulating leukocyte surface IL-10R expression therefore does not seem to play a role in the pathogenesis of SLE or rheumatoid arthritis. Functional IL-10R studies would be of interest.
ISSN:0392-856X
1593-098X