The presence of partially 3- O-methylated mannogalactan from the fruit bodies of edible basidiomycetes Pleurotus ostreatus ‘ florida’ Berk. and Pleurotus ostreatoroseus Sing

• Published in: FEMS microbiology letters 2003-04, Vol.221 (1), p.119-124
• Main Authors: Rosado, Fábio R, Carbonero, Elaine R, Claudino, Rafaela F, Tischer, César A, Kemmelmeier, Carlos, Iacomini, Marcello
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Carbohydrate Conformation; Carbohydrate Sequence; fruiting bodies; Fundamental and applied biological sciences. Psychology; Galactans - analysis; Magnetic Resonance Spectroscopy; Mannans - analysis; Mannogalactan; Methylation; Microbiology; Miscellaneous; Molecular Sequence Data; Mycology; Pleurotus - chemistry; Pleurotus - growth & development; Pleurotus djamor; Pleurotus ostreatoroseus; Pleurotus ostreatus; Pleurotus ostreatus ‘ florida’; Polysaccharide; spectroscopy; thawing
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(03)00161-7

The partially 3- O-methylated mannogalactans were isolated from the fruiting bodies of edible basidiomycetes Pleurotus ostreatus ‘ florida’ Berk. and Pleurotus ostreatoroseus Sing. They were obtained via successive aqueous extraction, freeze thawing, and precipitation with Fehling solution and then investigated using 13C- and 1H-nuclear magnetic resonance spectroscopy (including COSY, TOCSY and HMQC techniques), methylation analysis and Smith degradation. The main chain consisted of (1→6)-linked α- D-galactopyranosyl residues containing 3- O-Me-α- D-galactopyranoses, a part of these units being substituted in the position O-2 with β- D-mannopyranose residues. The heteropolysaccharides found were similar with differences only in the levels of the 3- O-Me-α- D-galactopyranoses residues. The presence of partially 3- O-methylated mannogalactan appears to be typical of Pleurotus spp.