Altered Cellular Distribution of Hexokinase in Skeletal Muscle after Exercise

• Published in: International journal of sports medicine 1992-07, Vol.13 (5), p.436-438
• Main Authors: Van Houten, D. R., Davis, J. M., Meyers, D. M., Durstine, J. L.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Fatigue; Fundamental and applied biological sciences. Psychology; Hexokinase - metabolism; Male; Mitochondria, Muscle - enzymology; Mitochondria, Muscle - metabolism; Muscles - enzymology; Muscles - metabolism; Physical Exertion - physiology; Rats; Rats, Inbred Strains; Short Communications; Space life sciences; Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports
• ISSN: 0172-4622; 1439-3964
• DOI: 10.1055/s-2007-1021294

Abstract The purpose of this study was to determine the effects of a single period of swim exercise to exhaustion on the cellular distribution of hexokinase in rat skeletal muscle. The gastrocnemius muscle from male rats exercised (n = 16) to exhaustion or allowed to remain inactive (n = 14) was analyzed for hexokinase activity (HK) in the whole muscle homogenate and the mitochondrial-rich fraction. Although exercise produced a slight, non-significant, increase in HK activity in the whole muscle homogenate (p < 0.09), HK activity in the mitochondrial-rich fraction was significantly increased in the exercised muscle (p < 0.02). This shift in the cellular distribution of HK is towards the more active mitochondrial bound form and indicates an enhanced regulation of HK activity during physical exercise.