Bovine leukemia virus gene expression in vivo

The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched...

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Bibliographic Details
Published in:Journal of Virology 1992-10, Vol.66 (10), p.6223-6225
Main Authors: Haas, L. (Cornell University, Ithaca, NY), Divers, T, Casey, J.W
Format: Article
Language:English
Subjects:
AIDS/HIV
Base Sequence
Biological and medical sciences
Blotting, Southern
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE
Gene Expression
GENES
Genes, pX
Genes, Viral
Genetics
INFECCION
INFECTION
Leukemia Virus, Bovine - genetics
Microbiology
Molecular Sequence Data
Polymerase Chain Reaction
RETROVIRIDAE
RNA, Viral - metabolism
Transcription, Genetic
Virology
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Summary:The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as leukemia/lymphosarcoma develops
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.66.10.6223-6225.1992