Two forms of ribulose-1,5-bisphosphate carboxylase/oxygenase from Thiobacillus denitrificans

The autotrophic, sulfur-oxidizing bacterium Thiobacillus denitrificans possesses two forms of the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The form I and form II genes were isolated from a cosmid library using heterologous DNA probes. Restriction enzyme analysis...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1992-07, Vol.73 (1-2), p.111-119
Main Authors: English, R S, Williams, C A, Lorbach, S C, Shively, J M
Format: Article
Language:English
Subjects:
DNA Probes
DNA, Bacterial - analysis
Isoenzymes - chemistry
Isoenzymes - genetics
Multigene Family - genetics
Ribulose-Bisphosphate Carboxylase - chemistry
Ribulose-Bisphosphate Carboxylase - genetics
Thiobacillus - chemistry
Thiobacillus - enzymology
Thiobacillus - genetics
Transcription, Genetic
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The autotrophic, sulfur-oxidizing bacterium Thiobacillus denitrificans possesses two forms of the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The form I and form II genes were isolated from a cosmid library using heterologous DNA probes. Restriction enzyme analysis indicated that the genes are within 17 kbp of each other. Other Calvin cycle enzyme genes are not present. Analysis of T. denitrificans RNA indicated that the form I genes for the large and small subunits are co-transcribed with a length of 2800 nucleotides. The transcript for the form II gene is 1900 nucleotides in length.
ISSN:0378-1097