Comparison of standard capillary and chip separations of sodium dodecylsulfate–protein complexes

• Published in: Journal of Chromatography A 2003-03, Vol.990 (1), p.153-158
• Main Authors: Deyl, Zdenĕk, Mikšı́k, Ivan, Eckhardt, Adam
• Format: Article
• Language: English
• Subjects: Electrophoresis, Capillary - methods; Proteins; Sodium Dodecyl Sulfate - chemistry; Sodium dodecylsulfate; Spectrophotometry, Ultraviolet
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)01523-6

Conditions for converting a set of five standard proteins to electrochemically active sodium dodecylsulfate (SDS) complexes were worked out with the aim of using such complexes for conductivity detection with a a chip electrophoresis system. The results obtained were compared with standard capillary electrophoresis (37 cm (effective length 30 cm)×75 μm I.D. capillary, 10 kV, negative polarity at the inlet). The chip separations were run at 500 V per chip (100 V/cm) as compared to the standard capillary arrangement, which was run at 266.6 V/cm. For the capillary set-up the protein complexes were prepared in aqueous solution (Milli-Q water) made 10 m M with respect to SDS. If the SDS concentration was increased to 50 m M, the separation in the capillary was incomplete. On the other hand with the chip system both approaches yielded acceptable results. The chip separations were slightly (but not distinctly) shorter and offered better separations than the standard set-up. The concentration of the surfactant used for the preparation the complexes results in alternations of the elution sequence, which is preserved if the chip separation is used instead of the capillary set-up. Apparently the full capacity of protein–SDS binding is not exploited for the preparation of the adducts.