A flow-cytometric method for determination of yeast viability and cell number in a brewery

A flow-cytometric assay, using the fluorescent dye, oxonol, for the simultaneous determination of yeast cell viability and cell number is described. The assay was optimised, and trialed at a brewery for 6 months. The flow-cytometry assay offered a substantially reduced error in viability determinati...

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Bibliographic Details
Published in:FEMS yeast research 2003-03, Vol.3 (1), p.11-16
Main Authors: Boyd, Andrew R, Gunasekera, Thusitha S, Attfield, Paul V, Simic, Kristina, Vincent, Scott F, Veal, Duncan A
Format: Article
Language:English
Subjects:
Cell Count
Flow cytometry
Flow Cytometry - methods
Fluorescent Dyes - analysis
Methylene Blue - pharmacology
Oxonol
Pitching rate
Reproducibility of Results
Saccharomyces carlsbergensis
Staining and Labeling - methods
Viability
Wine - microbiology
Yeasts - cytology
Yeasts - drug effects
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Summary:A flow-cytometric assay, using the fluorescent dye, oxonol, for the simultaneous determination of yeast cell viability and cell number is described. The assay was optimised, and trialed at a brewery for 6 months. The flow-cytometry assay offered a substantially reduced error in viability determination, compared to methylene blue which is the industry standard for measuring viability. Further, by calculating yeast cell number at the same time, this assay provides a reliable method for determining pitching rate, allowing increased quality control of subsequent fermentations.
ISSN:1567-1356
1567-1364
DOI:10.1016/S1567-1356(02)00125-3