Gene probe assays on a fibre-optic evanescent wave biosensor

This report describes experiments to detect oligonucleotide hybridization at the surface of a fibre-optic evanescent wave biosensor. Conditions were optimized and the time course of hybridization reactions were found to be very rapid compared to conventional hybridization assays. Binding was easily...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1992, Vol.7 (7), p.487-493
Main Authors: Graham, C.R., Leslie, D., Squirrell, D.J.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Biosensing Techniques
biosensor
Biosensors
Biotechnology
DNA
DNA Probes
evanescent wave device
Fiber Optic Technology
fibre-optic
Fundamental and applied biological sciences. Psychology
gene probes
hybridization
Methods. Procedures. Technologies
Molecular Sequence Data
Nucleic Acid Hybridization
oligonucleotide
Oligonucleotides - genetics
total internal reflection fluorescence
Various methods and equipments
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Summary:This report describes experiments to detect oligonucleotide hybridization at the surface of a fibre-optic evanescent wave biosensor. Conditions were optimized and the time course of hybridization reactions were found to be very rapid compared to conventional hybridization assays. Binding was easily reversed by heating and the sensor surface could be reused many times. Short (16- and 20-mer) oligonucleotides bound to the waveguide surface could be used to detect fluorescein-labelled complementary sequences at the nanomolar level. Polymerase chain reaction was used to generate a 204-base oligomer that was attached to the waveguide surface. Fluorescein-labelled oligos bound at either the proximal or distal ends of this probe were found to give similar outputs.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(92)80005-V