Activation of omental milky spots and milky spot macrophages by intraperitoneal administration of a streptococcal preparation, OK-432

Omental milky spots are omentum-associated lymphoid tissues that cede peritoneal macrophages and participate in the immunity of the peritoneal cavity. We studied the changing surface features of milky spots and milky spot macrophages of Wistar rats, following the i.p. administration of OK-432, a kil...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1992-10, Vol.52 (19), p.5400-5402
Main Authors: SHIMOTSUMA, M, SIMPSON-MORGAN, M. W, TAKAHASHI, T, HAGIWARA, A
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Immunologic Factors - pharmacology
Immunotherapy
Injections, Intraperitoneal
Lymphoid Tissue - cytology
Lymphoid Tissue - drug effects
Lymphoid Tissue - ultrastructure
Macrophages - drug effects
Macrophages - immunology
Macrophages - ultrastructure
Male
Medical sciences
Microscopy, Electron, Scanning
Omentum - physiology
Peritoneal Cavity - cytology
Pharmacology. Drug treatments
Picibanil - pharmacology
Rats
Rats, Wistar
Streptococcus
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Summary:Omental milky spots are omentum-associated lymphoid tissues that cede peritoneal macrophages and participate in the immunity of the peritoneal cavity. We studied the changing surface features of milky spots and milky spot macrophages of Wistar rats, following the i.p. administration of OK-432, a killed streptococcal preparation (1 Klinische Einheit (unit) in 5 ml of phosphate-buffered saline) by the use of scanning electron microscopy. OK-432-activated macrophages demonstrated marked surface membrane activity and migrated through the stomata of the milky spot into the peritoneal cavity. The characteristic features of activated milky spots and milky spot macrophages were noted as early as 3 h following the administration of OK-432, and continued to be observed until 7 days after the injection. By 14 days after the injection, the structural integrity of the milky spot was partially lost. The activation of milky spots and milky spot macrophages by OK-432 provides a convenient in vivo system for the monitoring and study of i.p. cellular events.
ISSN:0008-5472
1538-7445