Desiccation Tolerance of Spermatozoa Dried at Ambient Temperature: Production of Fetal Mice

Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from the B6D2F1 strain isolated in an EGTA-supplemented T...

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Bibliographic Details
Published in:Biology of reproduction 2003-05, Vol.68 (5), p.1779-1786
Main Authors: Bhowmick, Sankha, Zhu, Liben, McGinnis, Lynda, Lawitts, Joel, Nath, Bharat D, Toner, Mehmet, Biggers, John
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
Blastocyst - physiology
Buffers
Convection
Culture Media
Desiccation - methods
Edetic Acid - pharmacology
Embryo Implantation - physiology
Embryonic and Fetal Development - physiology
Female
Fluid Therapy
Freeze Drying
Gynecology. Andrology. Obstetrics
In Vitro Techniques
Insemination, Artificial - methods
Male
Medical sciences
Mice
Noble Gases
Oocytes - growth & development
Ovum - physiology
Pregnancy
Spermatozoa - physiology
Sterility. Assisted procreation
Temperature
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Summary:Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from the B6D2F1 strain isolated in an EGTA-supplemented Tris-HCl buffer were dried using three different drying rates and were stored for 18–24 h at 4°C. The mean final moisture content was
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.102.009407