Determination of catechins in human plasma after commercial canned green tea ingestion by high-performance liquid chromatography with electrochemical detection using a microbore column

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-05, Vol.788 (2), p.269-275
• Main Authors: Kotani, Akira, Miyashita, Naoto, Kusu, Fumiyo
• Format: Article
• Language: English
• Subjects: Adult; Antineoplastic agents; Biological and medical sciences; Catechin - blood; Catechins; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Electrochemistry - methods; General aspects; Humans; Male; Medical sciences; Pharmacology. Drug treatments; Reproducibility of Results; Tea - chemistry
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/S1570-0232(02)01036-X

Determination of catechins in human plasma was carried out by high-performance liquid chromatography with electrochemical detection using a microbore octadecylsilica column. Peak heights for catechins were found to be linearly related to the amount of each catechin injected, from 2 pmol/ml to 2 nmol/ml ( r>0.999). Conjugated-form catechins in plasma were hydrolyzed enzymatically using β-glucuronidase and sulfatase. Catechins in plasma and the hydrolyzed solution were extracted with ethyl acetate and determined by the present method. The time courses of concentrations of catechins in human plasma showed maxima at 1–2 h after ingestion of 340 ml of commercial canned green tea.