The effect of mechanical deformation on the distribution of potassium ions across the cell membrane of sutural cells

Ionic concentrations of potassium, sodium, and chloride were determined in osteocytes of the rat calvarium. The values were determined by fluorescent microscopy of both intra- and extracellular concentrations. Following the baseline determination, the calvaria were placed in tension by retraction of...

Full description

Saved in:
Bibliographic Details
Published in:Calcified tissue international 1992-06, Vol.50 (6), p.547-552
Main Authors: MCDONALD, F, HOUSTON, W. J. B
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone Remodeling - physiology
Cell Membrane - metabolism
Chlorides - metabolism
Diseases of the osteoarticular system
Medical sciences
Membrane Potentials
Microelectrodes
Microscopy, Fluorescence
Miscellaneous. Osteoarticular involvement in other diseases
Organ Culture Techniques
Osteocytes - metabolism
Potassium - metabolism
Potassium Channels - drug effects
Rats
Skull
Sodium - metabolism
Space life sciences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ionic concentrations of potassium, sodium, and chloride were determined in osteocytes of the rat calvarium. The values were determined by fluorescent microscopy of both intra- and extracellular concentrations. Following the baseline determination, the calvaria were placed in tension by retraction of a microelectrode manipulator, and the fluorescence of the cells were measured again. A statistically significant change in the derived ion distribution was found. Thus, the tensile forces affected the distribution of ions across the cell membranes, increasing intracellular sodium and decreasing intracellular potassium. This would have an effect on the resting cell membrane potential with a change of potential of 8 mV. This has implications in the interpretation of clinical findings.
ISSN:0171-967X
1432-0827
DOI:10.1007/BF00582171