Transient receptor potential (TRPC) channels in human sperm: expression, cellular localization and involvement in the regulation of flagellar motility

Capacitative Ca 2+ entry is a process whereby the activation of Ca 2+ influx through the plasma membrane is triggered by depletion of intracellular Ca 2+ stores. Some transient receptor potential (TRPC) proteins have been proposed as candidates for capacitative Ca 2+ channels. Recent evidence indica...

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Bibliographic Details
Published in:FEBS letters 2003-04, Vol.541 (1), p.69-74
Main Authors: Castellano, Laura E, Treviño, Claudia L, Rodrı́guez, Delany, Serrano, Carmen J, Pacheco, Judith, Tsutsumi, Vı́ctor, Felix, Ricardo, Darszon, Alberto
Format: Article
Language:English
Subjects:
Ca 2+ channel
Ca2+ channel
Calcium Channels - analysis
Calcium Channels - genetics
Calcium Channels - metabolism
Calcium Channels - physiology
Capacitative Ca 2+ entry
Capacitative Ca2+ entry
Fluorescent Antibody Technique, Indirect
Humans
Immunohistochemistry
Male
RNA, Messenger - biosynthesis
Sperm Motility
Spermatozoa - chemistry
Spermatozoa - metabolism
Spermatozoa - ultrastructure
Store operated channel
Transient receptor potential
TRPC
TRPC Cation Channels
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Summary:Capacitative Ca 2+ entry is a process whereby the activation of Ca 2+ influx through the plasma membrane is triggered by depletion of intracellular Ca 2+ stores. Some transient receptor potential (TRPC) proteins have been proposed as candidates for capacitative Ca 2+ channels. Recent evidence indicates that capacitative Ca 2+ entry participates in the sperm acrosome reaction (AR), an exocytotic process necessary for fertilization. In addition, several TRPCs have been detected heterogeneously distributed in mouse sperm, suggesting that they may participate in other functions such as motility. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA messengers for TRPC1, 3, 6 and 7 were found in human spermatogenic cells. Confocal indirect immunofluorescence revealed the presence of TRPC1, 3, 4 and 6 differentially localized in the human sperm, and immunogold transmission electron microscopy indicated that TRPC epitopes are mostly associated to the surface of the cells. Because all of them were detected in the flagellum, TRPC channel antagonists were tested in sperm motility using a computer-assisted assay. Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00305-3