KEDARCIDIN, A NEW CHROMOPROTEIN ANTITUMOR ANTIBIOTIC: II. ISOLATION, PURIFICATION AND PHYSICO-CHEMICAL PROPERTIES

Kedarcidin, a new chromoprotein antitumor antibiotic, was isolated from the culture broth of a novel actinomycete strain L585-6 (ATCC 53650). The antibiotic was recovered from the culture nitrate by adsorption to QAE ion exchanger and purified by successive application of gel filtration and ion exch...

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Bibliographic Details
Published in:Journal of antibiotics 1992/08/25, Vol.45(8), pp.1250-1254
Main Authors: HOFSTEAD, SANDRA J., MATSON, JAMES A., MALACKO, ALISON R., MARQUARDT, HANS
Format: Article
Language:English
Subjects:
Actinomycetales - metabolism
Alkynes - chemistry
Alkynes - isolation & purification
Amino Acid Sequence
Amino Acids - analysis
Anti-Bacterial Agents
Antibiotics, Antineoplastic - chemistry
Antibiotics, Antineoplastic - isolation & purification
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Applied microbiology
Biological and medical sciences
Chemotherapic agents
Chromatography, High Pressure Liquid
Cycloparaffins - chemistry
Cycloparaffins - isolation & purification
Enediynes
Fundamental and applied biological sciences. Psychology
Isoelectric Focusing
Microbiology
Molecular Sequence Data
Naphthalenes - chemistry
Naphthalenes - isolation & purification
Peptides
Proteins - chemistry
Proteins - isolation & purification
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Summary:Kedarcidin, a new chromoprotein antitumor antibiotic, was isolated from the culture broth of a novel actinomycete strain L585-6 (ATCC 53650). The antibiotic was recovered from the culture nitrate by adsorption to QAE ion exchanger and purified by successive application of gel filtration and ion exchange chromatography with Sephadex G-50 and DEAE-Sephadex, respectively. Kedarcidin is an acidic complex (pI 3.65) with an apparent molecular weight of 12, 400. The complex consists of a highly unstable, solvent extractable chromophore and a water soluble peptide. The apoprotein is a single chain polypeptide of 114 residues.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.45.1250