Coupling Capillary Electrochromatography with Electrospray Fourier Transform Mass Spectrometry for Characterizing Complex Oligosaccharide Pools

To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of microcolumn separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-04, Vol.75 (7), p.1684-1690
Main Authors: Que, Amy H, Mechref, Yehia, Huang, Yunping, Taraszka, John A, Clemmer, David E, Novotny, Milos V
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Cattle
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Chromatography - instrumentation
Chromatography - methods
Electrodes
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Exact sciences and technology
Fourier Analysis
Glycoproteins - chemistry
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Mucins - chemistry
Oligosaccharides - analysis
Oligosaccharides - chemistry
Other chromatographic methods
Scientific imaging
Spectrometric and optical methods
Sterol Esterase - chemistry
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Summary:To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of microcolumn separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from the mixtures of O-linked oligosaccharides originated from bovine mucin and bile salt-stimulated lipase, a large glycoprotein enzyme.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac025985c