A new ratiometric fluorescence probe as strong sensor of surface charge of lipid vesicles and micelles

We report on the ability of a new fluorescent probe, 4-(2-pyren-1-yl-vinyl) pyridine, 1, to respond to micelles and phospholipid vesicles of different surface charge. The probe gets incorporated into micellar and membranous assemblies and shows a large red-shift in the fluorescence emission maxima e...

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Bibliographic Details
Published in:FEBS letters 2003-04, Vol.541 (1), p.132-136
Main Authors: Singh, Yashveer, Gulyani, Akash, Bhattacharya, Santanu
Format: Article
Language:English
Subjects:
Anionic surface
Anions
CT, charge transfer
CTAB, cetyl trimethyl ammonium bromide
DCP, dicetyl phosphate
DPPA, dipalmitoylphosphatidic acid
DPPC, dipalmitoylphosphatidyl choline
Fluorescent Dyes - chemistry
Liposomes
Membrane surface charge
Membranes, Artificial
Micelle
Micelles
Phospholipid vesicle
Phospholipids - chemistry
Pyren-1-yl-vinyl pyridine
Pyridines - chemistry
Ratiometric probe
SDS, sodium dodecyl sulfate
Spectrometry, Fluorescence
Static Electricity
Tween® 20, polyoxyethylene sorbitan monolaureate
Vinyl Compounds - chemistry
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Summary:We report on the ability of a new fluorescent probe, 4-(2-pyren-1-yl-vinyl) pyridine, 1, to respond to micelles and phospholipid vesicles of different surface charge. The probe gets incorporated into micellar and membranous assemblies and shows a large red-shift in the fluorescence emission maxima especially when the surface charge of the organized media is anionic. The effect on the photo-excitation of the probe is very clear and pronounced as it can be easily visualized. The sample color upon photo-excitation changes from blue to orange/red once the probe experiences negatively charged vesicular or micellar surfaces. These results make the probe molecule useful as a reporter for sensing electrostatic environment in biological membranes and related organized assemblies.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00314-4