In vitro methods for culturing vertebrate and mosquito stages of Plasmodium

The development of in vitro culture systems for the vertebrate stages of Plasmodium led to major advancements in malaria research. Here we review both improvements made in these techniques and the recent achievement of the in vitro growth of mosquito stages from ookinete to infective sporozoite.

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Bibliographic Details
Published in:Microbes and Infection 2003-04, Vol.5 (4), p.321-327
Main Authors: Hurd, Hilary, Al-Olayan, Ebtesam, Butcher, Geoffrey A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Culture Techniques - methods
Culicidae
Culicidae - parasitology
Culture
Culture Media
Erythrocytes - parasitology
Erythrocytic stages
Fundamental and applied biological sciences. Psychology
Gametocytes
General aspects and techniques
Humans
Insect Vectors - parasitology
Life Cycle Stages
Malaria
Oocysts
Ookinetes
Parasitology - methods
Plasmodium
Plasmodium - growth & development
Protozoa
Reproduction, Asexual
Sporozoites
Sporozoites - growth & development
Tropical medicine
Vertebrates - parasitology
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Description
Summary:The development of in vitro culture systems for the vertebrate stages of Plasmodium led to major advancements in malaria research. Here we review both improvements made in these techniques and the recent achievement of the in vitro growth of mosquito stages from ookinete to infective sporozoite.
ISSN:1286-4579
1769-714X
DOI:10.1016/S1286-4579(03)00034-0