A Case of Bullous Pemphigoid Successfully Treated by Plasmapheresis: Assessment of the Change in Titers of Circulating Antibodies by Immunoblotting and Enzyme‐Linked Immunosorbent Assay

We report a case of bullous pemphigoid successfully treated with double filtration plasmapheresis. The changes in titers of circulating autoantibodies were assessed by immunoblotting and enzyme‐linked immunosorbent assay (ELISA) using a recombinant protein of the non‐collagenous 16a (NC16a) domain o...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dermatology 2003-04, Vol.30 (4), p.326-331
Main Authors: Lee, Jee Bum, Fumimori, Takeaki, Kurose, Koichiro, Mori, Osamu, Hashimoto, Takashi
Format: Article
Language:English
Subjects:
Autoantibodies - analysis
Autoantigens - analysis
bullous pemphigoid
Carrier Proteins
Collagen - analysis
Collagen Type XVII
Cytoskeletal Proteins
Dystonin
Enzyme-Linked Immunosorbent Assay
Follow-Up Studies
Humans
immunoblotting
Immunoblotting - methods
Male
Middle Aged
Nerve Tissue Proteins
Non-Fibrillar Collagens
Pemphigoid, Bullous - immunology
Pemphigoid, Bullous - therapy
plasmapheresis
Plasmapheresis - methods
Sensitivity and Specificity
Severity of Illness Index
Treatment Outcome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a case of bullous pemphigoid successfully treated with double filtration plasmapheresis. The changes in titers of circulating autoantibodies were assessed by immunoblotting and enzyme‐linked immunosorbent assay (ELISA) using a recombinant protein of the non‐collagenous 16a (NC16a) domain of the 180 kDa bullous pemphigoid antigen (BP180). The ELISA was shown to be more sensitive in detecting disease‐specific antoantibodies in the bullous pemphigoid sera. The reduction of titers of circulating autoantibodies in the sera correlated well with the decrease in the disease activity in both the first and second rounds of plasmapheresis treatment in this case.
ISSN:0385-2407
1346-8138
DOI:10.1111/j.1346-8138.2003.tb00395.x