Phylogenetically conserved amino acids of MBP and P0 from amphibian myelin

Myelin basic protein (MBP) and P0 glycoprotein are major structural proteins of myelin. In adult frog, MBP is found in both the central and peripheral nervous systems (CNS and PNS), while P0 is found exclusively in the PNS. To assess the phylogenetic conservation of these proteins, MBP and P0 were i...

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Bibliographic Details
Published in:Journal of molecular neuroscience 1992-01, Vol.3 (4), p.185-188
Main Authors: Karthigasan, J, Bauer, T K, Teplow, D B, Saavedra, R A, Kirschner, D A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Molecular Sequence Data
Myelin Basic Protein - genetics
Myelin P0 Protein
Myelin Proteins - genetics
Phylogeny
Rana catesbeiana - genetics
Sequence Homology
Species Specificity
Vertebrates - genetics
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Summary:Myelin basic protein (MBP) and P0 glycoprotein are major structural proteins of myelin. In adult frog, MBP is found in both the central and peripheral nervous systems (CNS and PNS), while P0 is found exclusively in the PNS. To assess the phylogenetic conservation of these proteins, MBP and P0 were isolated from adult bull-frog. A cyanogen bromide cleavage peptide of MBP (8-26), and the amino-terminal region (1-20) and an endoproteinase Lys-C peptide (67-79) of P0 were sequenced and compared to those of other vertebrate species. Residues that were conserved among other vertebrate species were found also to be conserved in frog: MBP--Ala18, Ser19, Thr20, Asp22; P0--Ile1, Val3, Thr5, Val13, Gly14, Ser15, Val17, Leu19, Trp72, Val73, Gly74, Lys79. These residues are located within or adjacent to regions that have been postulated to form beta strands and to be essential to the folding and function of these proteins.
ISSN:0895-8696
1559-1166
DOI:10.1007/BF03380137