New Turf for CFP/YFP FRET Imaging of Membrane Signaling Molecules

TIRF microscopy can be used in conjunction with CFP/YFP FRET to detect movements of the cytoplasmic tails of GIRK channels (Riven et al., this issue of Neuron). This innovative combination of techniques allows molecular resolution of small motions underlying ion channel activation by G proteins and...

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Bibliographic Details
Published in:Neuron 2003-04, Vol.38 (2), p.145-147
Main Authors: Evans, Jenafer, Yue, David T
Format: Article
Language:English
Subjects:
Bacterial Proteins - metabolism
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
Green Fluorescent Proteins
Labeling
Luminescent Proteins - metabolism
Microscopy
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Potassium Channels - metabolism
Proteins
Signal Transduction - physiology
Studies
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Summary:TIRF microscopy can be used in conjunction with CFP/YFP FRET to detect movements of the cytoplasmic tails of GIRK channels (Riven et al., this issue of Neuron). This innovative combination of techniques allows molecular resolution of small motions underlying ion channel activation by G proteins and will likely find widespread use for study of membrane-associated molecules.
ISSN:0896-6273
1097-4199
DOI:10.1016/S0896-6273(03)00234-4