Expression of hypothetical proteins in human fetal brain: increased expression of hypothetical protein 28.5kDa in Down syndrome, a clue for its tentative role

Major advances have been made in annotation of sequences of the human genome, although elucidating the functions of these newly discovered genes remains to be a strong challenge. In an effort to give insight into how triplication of chromosome 21 leads to mental retardation in Down syndrome, we have...

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Bibliographic Details
Published in:Molecular genetics and metabolism 2003-04, Vol.78 (4), p.295-301
Main Authors: Engidawork, Ephrem, Gulesserian, Talin, Fountoulakis, Michael, Lubec, Gert
Format: Article
Language:English
Subjects:
Brain - embryology
Chromosomes, Human, Pair 21 - genetics
Down Syndrome - genetics
Down Syndrome - metabolism
Electrophoresis, Gel, Two-Dimensional
Female
Fetus - metabolism
Humans
Male
Protein Biosynthesis
Protein Structure, Tertiary
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Major advances have been made in annotation of sequences of the human genome, although elucidating the functions of these newly discovered genes remains to be a strong challenge. In an effort to give insight into how triplication of chromosome 21 leads to mental retardation in Down syndrome, we have constructed a two-dimensional protein map from control and Down syndrome fetal brain and identified hypothetical proteins with no known functions. Subsequent quantitative analysis of these proteins revealed no apparent change in expression of hypothetical proteins DKZp564P0562.1 (fragment), 16.6, 21.4, 39.5, and 40kDa as well as putative 55kDa protein between controls and Down syndrome fetuses. By contrast, hypothetical protein 28.5kDa was significantly elevated (P
ISSN:1096-7192
DOI:10.1016/s1096-7192(03)00046-5