Analysis of Clonotype Distribution and Persistence for an Influenza Virus-Specific CD8 + T Cell Response

The spectrum of TCR Vβ usage is compared for primary and recall CD8 +D bPA 224 + T cell responses in mice with influenza pneumonia. Single-cell RT-PCR established that the same clonotypes were present in the lymphoid tissue and in the virus-infected lung. Longitudinal analysis indicated that the mem...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 2003-04, Vol.18 (4), p.549-559
Main Authors: Turner, Stephen J, Diaz, Gabriela, Cross, Richard, Doherty, Peter C
Format: Article
Language:English
Subjects:
Animals
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Epitopes, T-Lymphocyte
Female
Immunologic Memory
Influenza A virus - immunology
Lung - immunology
Lymphocytes
Mice
Mice, Inbred C57BL
Peptides
Receptors, Antigen, T-Cell, alpha-beta - analysis
Spleen
Spleen - immunology
T cell receptors
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Summary:The spectrum of TCR Vβ usage is compared for primary and recall CD8 +D bPA 224 + T cell responses in mice with influenza pneumonia. Single-cell RT-PCR established that the same clonotypes were present in the lymphoid tissue and in the virus-infected lung. Longitudinal analysis indicated that the memory TCR repertoire reflects the primary response, with no decrease in diversity prior to (or after) secondary challenge. The re-engagement of memory T cells looked to be stochastic in this localized, transient infection. Analysis of clonotypes from the blood, spleen, regional lymph nodes, bone marrow, lung, and liver over a 200 day interval showed no evidence of selective localization or loss. The long-term distribution of memory T cells seemed to be essentially random.
ISSN:1074-7613
1097-4180
DOI:10.1016/S1074-7613(03)00087-6