Equine Protozoal Myelitis in Panamanian Horses and Isolation of Sarcocystis neurona

Schizonts of Sarcocystis neurona were identified microscopically in hematoxylin-eosin-stained spinal cord sections from 2 native Panamanian horses that exhibited clinical signs of equine protozoal myelitis (EPM). Spinal cord homogenate from a third Panamanian horse with EPM was inoculated onto monol...

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Bibliographic Details
Published in:The Journal of parasitology 1992-10, Vol.78 (5), p.909-912
Main Authors: Granstrom, D. E., Alvarez, O., Dubey, J. P., Comer, P. F., Williams, N. M.
Format: Article
Language:English
Subjects:
Animal protozoal diseases
Animals
Antiserum
Biological and medical sciences
Cell Line
Encephalomyelitis
Female
Horse Diseases - epidemiology
Horse Diseases - parasitology
Horse Diseases - pathology
Horses
Immunoenzyme Techniques
Immunohistochemistry
Infectious diseases
Lesions
Male
Medical sciences
Merozoites
Microscopy, Electron
Myelitis - epidemiology
Myelitis - parasitology
Myelitis - pathology
Myelitis - veterinary
Panama - epidemiology
Parasites
Parasitic diseases
Parasitology
Protozoal diseases
Research Notes
Sarcocystis - isolation & purification
Sarcocystis - ultrastructure
Sarcocystis neurona
Sarcocystosis - epidemiology
Sarcocystosis - parasitology
Sarcocystosis - pathology
Sarcocystosis - veterinary
Schizonts
Spinal cord
Spinal Cord - parasitology
Spinal Cord - pathology
Veterinary medicine
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Summary:Schizonts of Sarcocystis neurona were identified microscopically in hematoxylin-eosin-stained spinal cord sections from 2 native Panamanian horses that exhibited clinical signs of equine protozoal myelitis (EPM). Spinal cord homogenate from a third Panamanian horse with EPM was inoculated onto monolayers of cultured bovine monocytes (M617). Intracytoplasmic schizonts containing merozoites arranged in rosette forms surrounding a central residual body first were observed 13 wk postinoculation. Parasites divided by endopolygeny and lacked rhoptries. Schizonts from each horse reacted with Sarcocystis cruzi antiserum in an immunohistochemical test.
ISSN:0022-3395
1937-2345
DOI:10.2307/3283328