Odontoblast transport of sulphate—the in vitro influence of fluoride

• Published in: Archives of oral biology 2003-05, Vol.48 (5), p.377-387
• Main Authors: Milan, A.M., Waddington, R.J., Smith, P.M., Embery, G.
• Format: Article
• Language: English
• Subjects: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology; Animals; Biological Transport; Cell Culture Techniques - methods; Chloride Channels - antagonists & inhibitors; Dentistry; Fluoride; Fluorides - pharmacology; Glycosaminoglycans; Male; Odontoblast; Odontoblasts - metabolism; Proteoglycans; Rats; Rats, Wistar; Sulfates - pharmacokinetics; Sulfur Radioisotopes - metabolism; Sulphate
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/S0003-9969(03)00016-5

The present study reports the development of a culture system for the analysis of 35 S -sulphate release from odontoblasts in vitro. Pulpless longitudinally split rat incisors were cultured in supplemented minimum essential medium (αMEM) with 20 μCi 35 S -sulphate per ml, 20 μCi 3 H -mannitol per ml for 1 h. Teeth were then transferred to fresh unlabelled media and aliquots of media were removed and the level of 35 S -sulphate 3 H -mannitol determined. Results indicated a two phase release of 35 S -sulphate into the media, and comparison with pulp tissue indicated a specific release pattern. Transport of sulphate is essential for correct synthesis and glycosylation of macromolecules such as proteoglycans (PG). Previous studies have shown that post-translational modifications of these proteins can be influenced by excess fluoride, resulting in decreased sulphation and elongation of glycosaminoglycan (GAG) chains. Therefore the influence of fluoride on sulphate transport, using the optimised culture system was also investigated. Inclusion of 6 mM fluoride during pulse labelling caused a significant decrease of 35 S -sulphate ( P