Assessment of the tissue distribution of transplanted human endothelial progenitor cells by radioactive labeling

Transplantation of endothelial progenitor cells (EPCs) improves vascularization and left ventricular function after experimental myocardial ischemia. However, tissue distribution of transplanted EPCs has not yet been monitored in living animals. Therefore, we tested whether radioactive labeling allo...

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Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 2003-04, Vol.107 (16), p.2134-2139
Main Authors: AICHER, Alexandra, BRENNER, Winfried, ZUHAYRA, Maaz, BADORFF, Cornel, MASSOUDI, Schirin, ASSMUS, Birgit, ECKEY, Thomas, HENZE, Eberhard, ZEIHER, Andreas M, DIMMELER, Stefanie
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cardiology. Vascular system
Cells, Cultured
Coronary heart disease
Endothelium, Vascular - cytology
Female
Fluorescence
Fluorescent Antibody Technique
Heart
Heart Ventricles
Humans
Indium Radioisotopes - administration & dosage
Indium Radioisotopes - analysis
Injections
Injections, Intravenous
Lipoproteins, LDL - chemistry
Medical sciences
Myocardial Infarction - therapy
Myocardium - cytology
Organometallic Compounds - administration & dosage
Organometallic Compounds - analysis
Oxyquinoline - administration & dosage
Oxyquinoline - analogs & derivatives
Oxyquinoline - analysis
Rats
Rats, Nude
Stem Cell Transplantation
Tissue Distribution
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Summary:Transplantation of endothelial progenitor cells (EPCs) improves vascularization and left ventricular function after experimental myocardial ischemia. However, tissue distribution of transplanted EPCs has not yet been monitored in living animals. Therefore, we tested whether radioactive labeling allows us to detect injected EPCs. Human EPCs were isolated from peripheral blood, characterized by expression of endothelial marker proteins, and radioactively labeled with [111In]indium oxine. EPCs (106) were injected in athymic nude rats 24 hours after myocardial infarction (n=8) or sham operation (n=8). Scintigraphic images were acquired after 1, 24, 48, and 96 hours after EPC injection. Animals were then killed, and specific radioactivity was measured in different tissues. At 24 to 96 hours after intravenous injection of EPCs, approximately 70% of the radioactivity was localized in the spleen and liver, with only approximately 1% of the radioactivity identified in the heart of sham-operated animals. After myocardial infarction, the heart-to-muscle radioactivity ratio increased significantly, from 1.02+/-0.19 in sham-operated animals to 2.03+/-0.37 after intravenous administration of EPCs. Injection of EPCs into the left ventricular cavity increased this ratio profoundly, from 2.69+/-1.54 in sham-operated animals to 4.70+/-1.55 (P
ISSN:0009-7322
1524-4539
DOI:10.1161/01.CIR.0000062649.63838.C9