Platelet-derived Growth Factor (PDGF)-C, a PDGF Family Member with a Vascular Endothelial Growth Factor-like Structure

Platelet-derived growth factor (PDGF)-C is a novel member of the PDGF family that binds to PDGF αα and αऔ receptors. The growth factor domain of PDGF-C (GFD-PDGF-C) was expressed in high yields in Escherichia coli and was purified and refolded from inclusion bodies obtaining a biologically active gr...

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Bibliographic Details
Published in:The Journal of biological chemistry 2003-05, Vol.278 (19), p.17114-17120
Main Authors: Reigstad, Laila J., Sande, Hege M., Fluge, Øystein, Bruland, Ove, Muga, Arturo, Varhaug, Jan Erik, Martı́nez, Aurora, Lillehaug, Johan R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Angiogenesis Inducing Agents - analysis
Angiogenesis Inducing Agents - chemistry
Angiogenesis Inducing Agents - genetics
Circular Dichroism
Escherichia coli
Humans
Lymphokines
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Sequence Data
Platelet-Derived Growth Factor - analysis
Platelet-Derived Growth Factor - chemistry
Platelet-Derived Growth Factor - genetics
Protein Conformation
Protein Structure, Tertiary - genetics
Recombinant Proteins - analysis
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Sequence Alignment
Structure-Activity Relationship
Vascular Endothelial Growth Factor A
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Summary:Platelet-derived growth factor (PDGF)-C is a novel member of the PDGF family that binds to PDGF αα and αऔ receptors. The growth factor domain of PDGF-C (GFD-PDGF-C) was expressed in high yields in Escherichia coli and was purified and refolded from inclusion bodies obtaining a biologically active growth factor with dimeric structure. The GFD-PDGF-C contains 12 cysteine residues, and Ellman assay analysis indicates that it contains three intramonomeric disulfide bonds, which is in accordance with GFD-PDGF-C being a member of the cystine knot superfamily of growth factors. The recombinant GFD-PDGF-C was characterized by CD, fluorescence, NMR, and infrared spectroscopy. Together, our data indicate that GFD-PDGF-C is a highly thermostable protein that contains mostly औ-sheet secondary structure and some (67) α-helix structure. The structural model of PDGF-C, obtained by homology-based molecular modeling using the structural representatives of this family of growth factors, shows that GFD-PDGF-C has a higher structural homology to the vascular endothelial growth factor than to PDGF-B. The modeled structure can give further insights into the function and specificity of this molecule.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M301728200