Isolation and characterisation of Shiga toxin-producing Escherichia coli O157:H7 from calves in Argentina

Four hundred and twenty-two calves were examined for intestinal carriage of Shiga toxin-producing Escherichia coli O157:H7 using conventional plating. Two (0.5%) E. coli O157 were recovered. They were compared with 96 Argentine strains of different origin by pulsed-field gel electrophoresis, phage t...

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Bibliographic Details
Published in:Research in veterinary science 2003-06, Vol.74 (3), p.283-286
Main Authors: Chinen, I, Otero, J.L, Miliwebsky, E.S, Roldán, M.L, Baschkier, A, Chillemi, G.M, Nóboli, C, Frizzo, L, Rivas, M
Format: Article
Language:English
Subjects:
Animals
Argentina - epidemiology
Bacteriophage Typing - veterinary
Beef
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - microbiology
Clonal relatedness
Cluster Analysis
Dairy products
Disease control
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
E coli
Electrophoresis, Gel, Pulsed-Field - veterinary
Escherichia coli Infections - epidemiology
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Escherichia coli O157 - genetics
Escherichia coli O157 - isolation & purification
Escherichia coli O157 - metabolism
Feces - microbiology
Genes
Haemolytic uraemic syndrome
Laboratories
Methods
Microbiology
Polymerase Chain Reaction - veterinary
Polymorphism, Restriction Fragment Length
Prevalence
Shiga Toxins - biosynthesis
STEC O157:H7
Studies
Veterinary medicine
Virulence factors
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Description
Summary:Four hundred and twenty-two calves were examined for intestinal carriage of Shiga toxin-producing Escherichia coli O157:H7 using conventional plating. Two (0.5%) E. coli O157 were recovered. They were compared with 96 Argentine strains of different origin by pulsed-field gel electrophoresis, phage typing and PCR-RFLP of stx2 genes. One strain isolated from a calf, was closely related with 18 strains of clinical origin.
ISSN:0034-5288
1532-2661
DOI:10.1016/S0034-5288(02)00192-3