Determinants of HIV-Specific CD8 T-cell responses in HIV-infected pediatric patients and enhancement of HIV-gag-specific responses with exogenous IL-15

Cellular immune responses play a central role in controlling HIV-1 infection. HIV-specific IFN-γ production by CD8 T cells was evaluated in 17 HLA-A2+ HIV-infected pediatric patients (age range 1 month to 16 years) in an ELISPOT assay. Most patients (15/17) exhibited responses to HIV-gag, followed b...

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Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2003-04, Vol.107 (1), p.36-45
Main Authors: Chitnis, Vivek, Pahwa, Rajendra, Pahwa, Savita
Format: Article
Language:English
Subjects:
Adolescent
Amino Acid Sequence
Antiviral Agents - immunology
Biological and medical sciences
CD4 Lymphocyte Count
CD8-Positive T-Lymphocytes - drug effects
CD8-Positive T-Lymphocytes - immunology
Child
Child, Preschool
Cohort Studies
Epitopes, T-Lymphocyte - immunology
Gene Products, gag - immunology
HIV Antigens - immunology
HIV Infections - immunology
HIV-1 - immunology
Human viral diseases
Humans
Infant
Infectious diseases
Interferon-gamma - biosynthesis
Interferon-gamma - immunology
Interleukin-15 - pharmacology
Medical sciences
Molecular Sequence Data
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Load
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Summary:Cellular immune responses play a central role in controlling HIV-1 infection. HIV-specific IFN-γ production by CD8 T cells was evaluated in 17 HLA-A2+ HIV-infected pediatric patients (age range 1 month to 16 years) in an ELISPOT assay. Most patients (15/17) exhibited responses to HIV-gag, followed by responses to envelope gp120, gp41, and V3 loop. Only 7 patients responded to all four antigenic peptides. Treatment-related immune reconstitution of CD4 T cells was associated with increase in gag-specific responses, but these declined with prolonged viral suppression. Exogenous IL-15 resulted in augmentation of HIV-gag-specific response in 71% of patients, while IL-2 and IL-7 had variable effects, augmenting responses in 25% patients. Thus, HIV-specific CD8 T-cell responses are dependent on both CD4 T-cell help and antigenic stimulation. The cytokine IL-15 may be a useful modality as adjunctive therapy to augment HIV-specific memory CD8 T cells.
ISSN:1521-6616
1521-7035
DOI:10.1016/S1521-6616(02)00051-7