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Physical exercise increases urinary excretion of lipoxin A4 and related compounds
1 Department of Human Pathology, and 5 Experimental and Clinical Department of Medicine and Pharmacology, University of Messina, 98125 Messina; 2 Center of Excellence on Aging, and Departments of 3 Biomedical Science and 4 Medicine and Aging, University "G. D'Annunzio," 66013 Chi...
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| Published in: | Journal of applied physiology (1985) 2003-06, Vol.94 (6), p.2237-2240 |
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| Main Authors: | , , , , , , , |
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| Language: | English |
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| container_end_page | 2240 |
| container_issue | 6 |
| container_start_page | 2237 |
| container_title | Journal of applied physiology (1985) |
| container_volume | 94 |
| creator | Gangemi, Sebastiano Luciotti, Graziella D'Urbano, Etrusca Mallamace, Agostino Santoro, Domenico Bellinghieri, Guido Davi, Giovanni Romano, Mario |
| description | 1 Department of Human Pathology, and
5 Experimental and Clinical Department of Medicine and
Pharmacology, University of Messina, 98125 Messina;
2 Center of Excellence on Aging, and Departments of
3 Biomedical Science and 4 Medicine and
Aging, University "G. D'Annunzio," 66013 Chieti,
Italy
Lipoxins (LX) are
lipoxygenase-derived eicosanoids with potent anti-inflammatory
activities and vascular bed-dependent vasodilatory actions. LX can be
formed in vitro and in vivo in a number of conditions, and we have
reported that immunoreactive LXA 4 (iLXA 4 ) is
physiologically excreted with human urine. Using a recently developed
LX extraction method coupled to an ELISA, we examined whether
iLXA 4 excretion was modified by strenuous exercise, which is known to trigger potential LX-forming events. Maximal exertion significantly increased iLXA 4 urinary excretion in nine
healthy volunteers (0.061 ± 0.023 vs. 0.113 ± 0.057 ng/mg
creatinine; P = 0.028). iLXA 4 levels
returned to baseline after 6 h and increased, although at a
smaller extent, after 24 h. A significant correlation ( r = 0.988) was denoted between iLXA 4 ELISA
measurements and reversed-phase high-performance liquid chromatography
quantitation of a previously described urinary tetraene, confirming its
LXA 4 -related nature. These findings show for the first time
that an increase in excretion of LXA 4 -related compounds can
be observed in response to strenuous exercise. This may be the
reflection of an enhanced LX biosynthesis, which may represent a
safeguard mechanism that keeps the inflammatory reaction triggered by
physical stress under control.
arachidonic acid; lipoxygenase; inflammation; metabolism |
| doi_str_mv | 10.1152/japplphysiol.01004.2002 |
| format | article |
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5 Experimental and Clinical Department of Medicine and
Pharmacology, University of Messina, 98125 Messina;
2 Center of Excellence on Aging, and Departments of
3 Biomedical Science and 4 Medicine and
Aging, University "G. D'Annunzio," 66013 Chieti,
Italy
Lipoxins (LX) are
lipoxygenase-derived eicosanoids with potent anti-inflammatory
activities and vascular bed-dependent vasodilatory actions. LX can be
formed in vitro and in vivo in a number of conditions, and we have
reported that immunoreactive LXA 4 (iLXA 4 ) is
physiologically excreted with human urine. Using a recently developed
LX extraction method coupled to an ELISA, we examined whether
iLXA 4 excretion was modified by strenuous exercise, which is known to trigger potential LX-forming events. Maximal exertion significantly increased iLXA 4 urinary excretion in nine
healthy volunteers (0.061 ± 0.023 vs. 0.113 ± 0.057 ng/mg
creatinine; P = 0.028). iLXA 4 levels
returned to baseline after 6 h and increased, although at a
smaller extent, after 24 h. A significant correlation ( r = 0.988) was denoted between iLXA 4 ELISA
measurements and reversed-phase high-performance liquid chromatography
quantitation of a previously described urinary tetraene, confirming its
LXA 4 -related nature. These findings show for the first time
that an increase in excretion of LXA 4 -related compounds can
be observed in response to strenuous exercise. This may be the
reflection of an enhanced LX biosynthesis, which may represent a
safeguard mechanism that keeps the inflammatory reaction triggered by
physical stress under control.
arachidonic acid; lipoxygenase; inflammation; metabolism</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/japplphysiol.01004.2002</identifier><identifier>PMID: 12588790</identifier><language>eng</language><publisher>United States: Am Physiological Soc</publisher><subject>Acids ; Adult ; Chromatography, High Pressure Liquid ; Enzyme-Linked Immunosorbent Assay ; Enzymes ; Exercise ; Exercise - physiology ; Female ; Humans ; Hydroxyeicosatetraenoic Acids - urine ; Lipoxins ; Male ; Metabolism ; Physical Endurance ; Reference Values ; Time Factors ; Urine</subject><ispartof>Journal of applied physiology (1985), 2003-06, Vol.94 (6), p.2237-2240</ispartof><rights>Copyright American Physiological Society Jun 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12588790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gangemi, Sebastiano</creatorcontrib><creatorcontrib>Luciotti, Graziella</creatorcontrib><creatorcontrib>D'Urbano, Etrusca</creatorcontrib><creatorcontrib>Mallamace, Agostino</creatorcontrib><creatorcontrib>Santoro, Domenico</creatorcontrib><creatorcontrib>Bellinghieri, Guido</creatorcontrib><creatorcontrib>Davi, Giovanni</creatorcontrib><creatorcontrib>Romano, Mario</creatorcontrib><title>Physical exercise increases urinary excretion of lipoxin A4 and related compounds</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>1 Department of Human Pathology, and
5 Experimental and Clinical Department of Medicine and
Pharmacology, University of Messina, 98125 Messina;
2 Center of Excellence on Aging, and Departments of
3 Biomedical Science and 4 Medicine and
Aging, University "G. D'Annunzio," 66013 Chieti,
Italy
Lipoxins (LX) are
lipoxygenase-derived eicosanoids with potent anti-inflammatory
activities and vascular bed-dependent vasodilatory actions. LX can be
formed in vitro and in vivo in a number of conditions, and we have
reported that immunoreactive LXA 4 (iLXA 4 ) is
physiologically excreted with human urine. Using a recently developed
LX extraction method coupled to an ELISA, we examined whether
iLXA 4 excretion was modified by strenuous exercise, which is known to trigger potential LX-forming events. Maximal exertion significantly increased iLXA 4 urinary excretion in nine
healthy volunteers (0.061 ± 0.023 vs. 0.113 ± 0.057 ng/mg
creatinine; P = 0.028). iLXA 4 levels
returned to baseline after 6 h and increased, although at a
smaller extent, after 24 h. A significant correlation ( r = 0.988) was denoted between iLXA 4 ELISA
measurements and reversed-phase high-performance liquid chromatography
quantitation of a previously described urinary tetraene, confirming its
LXA 4 -related nature. These findings show for the first time
that an increase in excretion of LXA 4 -related compounds can
be observed in response to strenuous exercise. This may be the
reflection of an enhanced LX biosynthesis, which may represent a
safeguard mechanism that keeps the inflammatory reaction triggered by
physical stress under control.
arachidonic acid; lipoxygenase; inflammation; metabolism</description><subject>Acids</subject><subject>Adult</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Enzymes</subject><subject>Exercise</subject><subject>Exercise - physiology</subject><subject>Female</subject><subject>Humans</subject><subject>Hydroxyeicosatetraenoic Acids - urine</subject><subject>Lipoxins</subject><subject>Male</subject><subject>Metabolism</subject><subject>Physical Endurance</subject><subject>Reference Values</subject><subject>Time Factors</subject><subject>Urine</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNp1kVFvFCEUhYnR2LX6F5T4YPoyW2BggMemsdWkSW1SnwkMd7ps2GGEmbj772XT1QeT3heSe757LhwQ-kTJmlLBLrd2muK0OZSQ4ppQQviaEcJeoVVVWUM7Ql-jlZKCNFIoeYbelbIlhHIu6Ft0RplQSmqyQg8_jia9jRj2kPtQAIexz2ALFLzkMNp8qFLtzCGNOA04hintw4ivOLajxxmincHjPu2mtIy-vEdvBhsLfDid5-jnzdfH62_N3f3t9-uru2bDdDs3jmk-KNoO4Li0Dpz2inLXCdYxx7ylvleM6F5oIhlo57nwonW26tBqTtpz9OXZd8rp1wJlNrtQeojRjpCWYmTLOiqZrODn_8BtWvJY72bYsYhSqkIfT9DiduDNlMOuPt38TaoCF8_AJjxtfocM5hR_ejqY-h1Gc9NVw_a4kL-M3iwxPsJ-Ps78GzGTH9o_4NOPoA</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>Gangemi, Sebastiano</creator><creator>Luciotti, Graziella</creator><creator>D'Urbano, Etrusca</creator><creator>Mallamace, Agostino</creator><creator>Santoro, Domenico</creator><creator>Bellinghieri, Guido</creator><creator>Davi, Giovanni</creator><creator>Romano, Mario</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Physical exercise increases urinary excretion of lipoxin A4 and related compounds</title><author>Gangemi, Sebastiano ; Luciotti, Graziella ; D'Urbano, Etrusca ; Mallamace, Agostino ; Santoro, Domenico ; Bellinghieri, Guido ; Davi, Giovanni ; Romano, Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h293t-b294f813feb47abeb9d814b65262b2da1dc8209c59072e9bd45d53ba526e39403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acids</topic><topic>Adult</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Enzymes</topic><topic>Exercise</topic><topic>Exercise - physiology</topic><topic>Female</topic><topic>Humans</topic><topic>Hydroxyeicosatetraenoic Acids - urine</topic><topic>Lipoxins</topic><topic>Male</topic><topic>Metabolism</topic><topic>Physical Endurance</topic><topic>Reference Values</topic><topic>Time Factors</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gangemi, Sebastiano</creatorcontrib><creatorcontrib>Luciotti, Graziella</creatorcontrib><creatorcontrib>D'Urbano, Etrusca</creatorcontrib><creatorcontrib>Mallamace, Agostino</creatorcontrib><creatorcontrib>Santoro, Domenico</creatorcontrib><creatorcontrib>Bellinghieri, Guido</creatorcontrib><creatorcontrib>Davi, Giovanni</creatorcontrib><creatorcontrib>Romano, Mario</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gangemi, Sebastiano</au><au>Luciotti, Graziella</au><au>D'Urbano, Etrusca</au><au>Mallamace, Agostino</au><au>Santoro, Domenico</au><au>Bellinghieri, Guido</au><au>Davi, Giovanni</au><au>Romano, Mario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physical exercise increases urinary excretion of lipoxin A4 and related compounds</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>94</volume><issue>6</issue><spage>2237</spage><epage>2240</epage><pages>2237-2240</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><abstract>1 Department of Human Pathology, and
5 Experimental and Clinical Department of Medicine and
Pharmacology, University of Messina, 98125 Messina;
2 Center of Excellence on Aging, and Departments of
3 Biomedical Science and 4 Medicine and
Aging, University "G. D'Annunzio," 66013 Chieti,
Italy
Lipoxins (LX) are
lipoxygenase-derived eicosanoids with potent anti-inflammatory
activities and vascular bed-dependent vasodilatory actions. LX can be
formed in vitro and in vivo in a number of conditions, and we have
reported that immunoreactive LXA 4 (iLXA 4 ) is
physiologically excreted with human urine. Using a recently developed
LX extraction method coupled to an ELISA, we examined whether
iLXA 4 excretion was modified by strenuous exercise, which is known to trigger potential LX-forming events. Maximal exertion significantly increased iLXA 4 urinary excretion in nine
healthy volunteers (0.061 ± 0.023 vs. 0.113 ± 0.057 ng/mg
creatinine; P = 0.028). iLXA 4 levels
returned to baseline after 6 h and increased, although at a
smaller extent, after 24 h. A significant correlation ( r = 0.988) was denoted between iLXA 4 ELISA
measurements and reversed-phase high-performance liquid chromatography
quantitation of a previously described urinary tetraene, confirming its
LXA 4 -related nature. These findings show for the first time
that an increase in excretion of LXA 4 -related compounds can
be observed in response to strenuous exercise. This may be the
reflection of an enhanced LX biosynthesis, which may represent a
safeguard mechanism that keeps the inflammatory reaction triggered by
physical stress under control.
arachidonic acid; lipoxygenase; inflammation; metabolism</abstract><cop>United States</cop><pub>Am Physiological Soc</pub><pmid>12588790</pmid><doi>10.1152/japplphysiol.01004.2002</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of applied physiology (1985), 2003-06, Vol.94 (6), p.2237-2240 |
| issn | 8750-7587 1522-1601 |
| language | eng |
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| source | American Physiological Society:Jisc Collections:American Physiological Society Journals ‘Read Publish & Join’ Agreement:2023-2024 (Reading list); American Physiological Society Free |
| subjects | Acids Adult Chromatography, High Pressure Liquid Enzyme-Linked Immunosorbent Assay Enzymes Exercise Exercise - physiology Female Humans Hydroxyeicosatetraenoic Acids - urine Lipoxins Male Metabolism Physical Endurance Reference Values Time Factors Urine |
| title | Physical exercise increases urinary excretion of lipoxin A4 and related compounds |
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