Identification of the bacterial etiology of culture-negative endocarditis by amplification and sequencing of a small ribosomal RNA gene

We report two cases of culture-negative bacterial endocarditis in which the organisms were identified by amplification and sequencing of the bacterial 16S rRNA gene. These results support an important role for polymerase chain reaction followed by direct sequencing to determine the etiology of cultu...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2003-05, Vol.46 (1), p.9-11
Main Authors: Khulordava, Irakli, Miller, Geraldine, Haas, David, Li, Haijing, McKinsey, Joel, Vanderende, Daniel, Tang, Y.i-Wei
Format: Article
Language:English
Subjects:
Adult
Anti-Bacterial Agents
Bacterial diseases
Bacterial endocarditis, myocarditis and pericarditis. Bacterial diseases of the aorta, limb vessels and lymphatic vessels
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Drug Therapy, Combination - administration & dosage
Echocardiography, Doppler
Endocarditis, Bacterial - diagnostic imaging
Endocarditis, Bacterial - drug therapy
Endocarditis, Bacterial - microbiology
False Negative Reactions
Female
Follow-Up Studies
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Microbiology
Middle Aged
Polymerase Chain Reaction - methods
Risk Assessment
RNA, Bacterial - analysis
RNA, Ribosomal, 16S - analysis
Sensitivity and Specificity
Severity of Illness Index
Streptococcus - classification
Streptococcus - genetics
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Description
Summary:We report two cases of culture-negative bacterial endocarditis in which the organisms were identified by amplification and sequencing of the bacterial 16S rRNA gene. These results support an important role for polymerase chain reaction followed by direct sequencing to determine the etiology of culture-negative bacterial endocarditis and to guide appropriate antimicrobial therapy.
ISSN:0732-8893
1879-0070
DOI:10.1016/S0732-8893(03)00011-7