PCR method for detection of largemouth bass virus

A polymerase chain reaction (PCR) method was developed for largemouth bass virus (LMBV). This iridovirus can cause a lethal disease of largemouth bass Micropterus salmoides, but also subclinically infects largemouth bass and other species of fishes. Oligonucleotide primers were designed to specifica...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2003-03, Vol.54 (1), p.29-33
Main Authors: GRIZZLE, John M, ALTINOK, Ilhan, NOYES, Andrew D
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Animals
Base Sequence
Bass
Biological and medical sciences
Capsid - chemistry
Capsid Proteins
DNA Virus Infections - diagnosis
DNA Virus Infections - veterinary
DNA Virus Infections - virology
DNA, Viral - chemistry
Fish Diseases - diagnosis
Fish Diseases - virology
Freshwater
Fundamental and applied biological sciences. Psychology
Gene Amplification
Iridoviridae
Iridovirus - genetics
Iridovirus - isolation & purification
Largemouth bass virus
Micropterus salmoides
Molecular Sequence Data
Pisciculture
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Sensitivity and Specificity
Vertebrate aquaculture
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Summary:A polymerase chain reaction (PCR) method was developed for largemouth bass virus (LMBV). This iridovirus can cause a lethal disease of largemouth bass Micropterus salmoides, but also subclinically infects largemouth bass and other species of fishes. Oligonucleotide primers were designed to specifically amplify the major capsid protein gene of LMBV. The protocol for sample processing and PCR provided a method that was more sensitive than cell culture for detection of LMBV in fish. The specific amplification of LMBV also provided an improved method for confirming the identity of cell-culture isolates presumptively identified as LMBV.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao054029